The location of silver in frog epidermis after treatment by ranvier's method, and possible implication of the flask cells in transport
The location of silver in frog epidermis after treatment by ranvier's method, and possible implication of the flask cells in transport
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朗飞法处理后青蛙表皮中银的位置，以及烧瓶细胞在运输中的可能意义
DOI：
10.1007/bf00307128
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Whitear
中科院分区：
文献类型：
--
作者：
M. Whitear
蛙皮肤，膀胱壁，和坐骨神经处理的Ranvier的银硝酸盐的方法，随后固定和切片的电子显微镜。在皮肤的表皮中，发现烧瓶细胞中沉积的银比其他地方多，特别是在烧瓶颈部的亚顶端斑块，其在皮肤用硝酸银充满5分钟后出现。膀胱中富含线粒体的细胞也比周围的上皮细胞积累更多的银，但不显示这种明显的亚顶端斑块。在类似处理的有髓神经纤维中，银积聚在轴突的结旁区域和轴突外的结处。有人建议，银可能会积累附近的一个网站的离子运输，由于结构的专业化不可见的标准电子显微镜技术，因此，烧瓶细胞可能涉及在青蛙皮肤中的运输。
Frog skin, bladder wall, and sciatic nerve were treated by Ranvier's silver nitrate method and subsequently fixed and sectioned for electron microscopy. In the epidermis of the skin, more silver is found deposited in the flask cells than elsewhere, especially as a sub-apical plaque in the neck of the flask, which appears after the skin has been flooded with silver nitrate for 5 minutes. Mitochondria rich cells in the bladder also accumulate more silver than the surrounding epithelial cells, but do not show such a distinct sub-apical plaque. In myelinated nerve fibres treated similarly, silver accumulates in the paranodal regions of the axon, and outside the axon at the node. It is suggested that silver may accumulate near a site of ion transport, due to structural specialisations not visible by standard electron microscope techniques, and that the flask cells may therefore be implicated in transport in the frog skin.