Genetic Diversity Among Viruses Associated with Sugarcane Mosaic Disease in Tucumaan, Argentina
Genetic Diversity Among Viruses Associated with Sugarcane Mosaic Disease in Tucumaan, Argentina
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DOI：
10.1094/phyto-99-1-0038
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发表时间：
2009-01-01
期刊：
影响因子：
3.2
通讯作者：
Castagnaro, A. P.
中科院分区：
文献类型：
--
作者：
Perera, M. F.;Filippone, M. P.;Castagnaro, A. P.
2006-07年在Tucumaan（阿根廷）收集了具有花叶症状的甘蔗叶片，并通过逆转录酶聚合酶链反应（RT-PCR）限制性片段长度多态性（RFLP）和甘蔗花叶病毒（SCMV）和高粱花叶病毒（SrMV）外壳蛋白（CP）基因片段的测序进行了分析。在96.6%的样品中检测到SCMV，其中41%的样品显示与菌株E的RFLP图谱一致。其余的样本产生了八种不同的谱，与其他已知的菌株不匹配。SCMV的分布似乎与甘蔗基因型的关系比与地理来源的关系更大，CP基因的序列分析显示了更大的遗传多样性与其他研究相比。在63.2%的样本中检测到SrMV，其中大多数也被SCMV感染，这表明，与其他国家和阿根廷其他省份不同，在图库曼，高水平的合并感染并不常见，共存是常见的。RFLP分析表明，SrMV株M（68%）和I（14%）的存在，而M和H株之间的共感染存在于18%的样品。未检测到SCMV其它亚组成员和甘蔗条斑花叶病毒（SCSMV）。我们的研究结果还表明，测序是目前唯一可靠的方法来评估SCMV和SrMV的遗传多样性，因为RT-PCR-RFLP可能不足以区分。
Sugarcane leaves with mosaic symptoms were collected in 2006-07 in Tucumaan (Argentina) and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) restriction fragment length polymorphism (RFLP) and sequencing of a fragment of the Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV) coat protein (CP) genes. SCMV was detected in 96.6% of samples, with 41% showing the RFLP profile consistent with strain E. The remaining samples produced eight different profiles that did not match other known strains. SCMV distribution seemed to be more related to sugarcane genotype than to geographical origin, and sequence analyses of CP genes showed a greater genetic diversity compared with other studies. SrMV was detected in 63.2% of samples and most of these were also infected by SCMV, indicating that, unlike other countries and other Argentinean provinces, where high levels of co-infection are infrequent, co-existence is common in Tucumaan. RFLP analysis showed the presence of SrMV strains M (68%) and I (14%), while co-infection between M and H strains was present in 18% of samples. Other SCMV subgroup members and the Sugarcane streak mosaic virus (SCSMV) were not detected. Our results also showed that sequencing is currently the only reliable method to assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may not be sufficiently discriminating.