Regulation of Prox 1 during lens regeneration.
Regulation of Prox 1 during lens regeneration.
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DOI：
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发表时间：
1999-08
影响因子：
4.4
通讯作者：
K. Del Rio-Tsonis;S. Tomarev;P. Tsonis
中科院分区：
文献类型：
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作者：
K. Del Rio-Tsonis;S. Tomarev;P. Tsonis
目的 确定 Prox 1 在无尾眼绿眼藻晶状体再生过程中的表达模式。方法进行聚合酶链式反应来扩增部分蝾螈Prox 1 序列。采用原位杂交和免疫检测方法分别检测Prox 1 mRNA和Prox 1蛋白。结果 Prox 1 mRNA 存在于完整眼睛的视网膜和晶状体（上皮和弓形区域）中。研究发现 Prox 1 蛋白主要存在于完整眼睛的晶状体和背侧虹膜中，尽管在腹侧虹膜中也检测到了一些痕量水平的 Prox 1 蛋白。晶状体切除术后，背侧去分化虹膜和再生晶状体中 mRNA 的表达也很明显。腹侧虹膜也表达 Prox 1，但似乎水平较低。尽管 Prox 1 蛋白在晶状体再生过程中在背侧虹膜中表现出上调，但在腹侧虹膜中也检测到了微量水平。在视网膜中，Prox 1蛋白分布在内核层的水平细胞中，而mRNA则在视网膜的所有层中表达。结论 Prox 1 在蝾螈虹膜的完整细胞中分布不均匀，背侧边缘的 Prox 1 蛋白水平显着高于腹侧边缘。该蛋白在晶状体再生过程中受到差异性调节，在虹膜背侧的调节明显上调。 Prox 1 是第一个被证明在晶状体再生过程中在虹膜背侧和腹侧虹膜中受到调节的转录因子。
PURPOSE To determine the expression pattern of Prox 1 during the process of lens regeneration in the urodele Notophthalmus viridescens. METHODS Polymerase chain reaction was performed to amplify a partial newt Prox 1 sequence. In situ hybridization and immunodetection methods were used to detect the Prox 1 mRNA and the Prox 1 protein, respectively. RESULTS Prox 1 mRNA was present in the retina and in the lens (in the epithelium and bow region) of the intact eye. Prox 1 protein was found to be predominantly present in the lens and dorsal iris of the intact eye, although some trace levels of Prox 1 protein were detected in the ventral iris as well. After lentectomy, expression of the mRNA was also pronounced in the dorsal dedifferentiating iris and the regenerating lens. The ventral iris also expressed Prox 1 but seemingly at lower levels. Although Prox 1 protein showed upregulation in the dorsal iris during the process of lens regeneration, trace levels were also detected in the ventral iris. In the retina, Prox 1 protein was distributed in horizontal cells of the inner nuclear layer, whereas the mRNA was expressed in all layers of the retina. CONCLUSIONS Prox 1 was unevenly distributed in the intact cells of the newt iris, with significantly higher levels of Prox 1 protein present in the dorsal versus the ventral margin. This protein was differentially regulated during the process of lens regeneration, with obvious upregulation in the dorsal iris. Prox 1 is the first transcriptional factor to be shown to be regulated in the dorsal versus ventral iris during the process of lens regeneration.