Alterations of MicroRNA Expression in the Liver, Heart, and Testis of Mice Upon Exposure to Repeated Low-Dose Radiation.
Alterations of MicroRNA Expression in the Liver, Heart, and Testis of Mice Upon Exposure to Repeated Low-Dose Radiation.
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DOI：
10.1177/1559325818799561
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Liang X;Zheng S;Cui J;Yu D;Yang G;Zhou L;Wang B;Cai L;Li W
微小RNA（miRs）在转录后水平调控靶基因表达，在高剂量电离辐射诱导生物效应中起关键作用。然而，体内反复低剂量辐射（LDR）后的miR表达谱尚未阐明。本研究调查了小鼠肝脏、心脏和睾丸在反复低剂量辐射暴露4个月后，11种在代谢、DNA损伤与修复、炎症和纤维化方面具有相关功能的miRs的反应谱。在低剂量辐射暴露后即刻以及2个月时，使用茎环定量逆转录聚合酶链反应评估表达谱。在这两个时间点，表达谱均有显著差异。在器官层面，心脏受影响最大，其次是肝脏和睾丸，其中发现与DNA损伤反应相关的miR显著上调。肝脏中与代谢相关的miRs下降，而睾丸中则上升。目前的结果显示，不同器官在反复低剂量辐射后，miR表达谱出现即刻和长期的改变。
MicroRNAs (miRs), which regulate target gene expression at the post-transcriptional level, play a crucial role in inducing biological effects upon high-dose ionizing radiation. Yet, the miR expression profiles in response to repeated low-dose radiation (LDR) in vivo have not been elucidated. This study investigated the response profiles of 11 miRs with functions involved in metabolism, DNA damage and repair, inflammation, and fibrosis in mouse liver, heart, and testis upon repeated LDR exposure for 4 months. The expression profiles were evaluated using stem-loop quantitative reverse transcription polymerase chain reaction immediately and at 2 months after LDR exposure. The expression profiles varied significantly at both time points. At the organ level, the heart was the most affected, followed by the liver and testis, in which significant miR upregulation related to DNA damage response was found. Metabolism-related miRs decreased in the liver and increased in the testis. The current results showed immediate and long-lasting alterations in the miR expression profiles in response to repeated LDR in different organs.
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