Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant
Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant
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DOI：
10.1126/science.289.5488.2338
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发表时间：
2000-09-29
期刊：
影响因子：
56.9
通讯作者：
Schroeder, JI
中科院分区：
文献类型：
--
作者：
Allen, GJ;Chu, SP;Schroeder, JI
在动物细胞中，胞质钙振荡控制信号传导，而在植物中，其重要性仍然是未知的。在野生型拟南芥保卫细胞脱落酸，氧化应激，冷，和外部钙引起不同幅度和频率的胞质钙振荡，诱导气孔关闭。在保卫细胞的V-ATP酶突变体det 3，外部钙和氧化应激引起长时间的钙增加，不振荡，气孔关闭被废除。相反，寒冷和脱落酸引起钙振荡det 3，气孔关闭正常发生。此外，在det 3保卫细胞，实验施加外部钙诱导的振荡拯救气孔关闭。这些数据提供了遗传证据，刺激特异性钙振荡气孔关闭是必要的。
Cytosolic calcium oscillations control signaling in animal cells, whereas in plants their importance remains Largely unknown. In wild-type Arabidopsis guard cells abscisic acid, oxidative stress, cold, and external calcium elicited cytosolic calcium oscillations of differing amplitudes and frequencies and induced stomatal closure. In guard cells of the V-ATPase mutant det3, external calcium and oxidative stress elicited prolonged calcium increases, which did not oscillate, and stomatal closure was abolished. Conversely, cold and abscisic acid elicited calcium oscillations in det3, and stomatal closure occurred normally. Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure.