Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity.
Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity.
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DOI：
10.1038/srep23449
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发表时间：
2016-03-21
影响因子：
4.6
通讯作者：
Preiser PR
中科院分区：
文献类型：
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作者：
Yam XY;Brugat T;Siau A;Lawton J;Wong DS;Farah A;Twang JS;Gao X;Langhorne J;Preiser PR
疟原虫多基因家族在疟疾的发病机制中起着核心作用。疟原虫散在重复序列（pir）基因是疟原虫属中最大的多基因家族。然而，它们的功能仍然未知。使用啮齿动物模型的疟疾，疟原虫夏氏，我们表明，个别CIR蛋白有差异的本地化内感染的红细胞（iRBC），这表明不同的功能作用，在血液阶段的感染。一些CIR似乎位于iRBC和裂殖子的表面上，因此很好地与宿主分子相互作用。根据这一假设，我们首次表明重组CIR的一个子集与小鼠RBC结合，这表明CIR在玫瑰花结形成和/或侵袭中发挥作用。总之，我们的研究结果揭示了cir家族成员之间在亚细胞定位和结合小鼠红细胞能力方面的差异，这有力地表明了在血液阶段感染中的不同功能作用。
Plasmodium multigene families play a central role in the pathogenesis of malaria. The Plasmodium interspersed repeat (pir) genes comprise the largest multigene family in many Plasmodium spp. However their function(s) remains unknown. Using the rodent model of malaria, Plasmodium chabaudi, we show that individual CIR proteins have differential localizations within infected red cell (iRBC), suggesting different functional roles in a blood-stage infection. Some CIRs appear to be located on the surface of iRBC and merozoites and are therefore well placed to interact with host molecules. In line with this hypothesis, we show for the first time that a subset of recombinant CIRs bind mouse RBCs suggesting a role for CIR in rosette formation and/or invasion. Together, our results unravel differences in subcellular localization and ability to bind mouse erythrocytes between the members of the cir family, which strongly suggest different functional roles in a blood-stage infection.