AN ADENOVIRUS VECTOR FOR GENE-TRANSFER INTO NEURONS AND GLIA IN THE BRAIN
AN ADENOVIRUS VECTOR FOR GENE-TRANSFER INTO NEURONS AND GLIA IN THE BRAIN
复制标题
DOI：
10.1126/science.8382374
复制
发表时间：
1993-02-12
期刊：
影响因子：
56.9
通讯作者：
MALLET, J
中科院分区：
文献类型：
--
作者：
LASALLE, GL;ROBERT, JJ;MALLET, J
将遗传物质有效地导入静止的神经细胞在脑功能的研究和神经疾病的基因治疗中是重要的。复制缺陷型腺病毒载体，含有报告基因编码β-半乳糖苷酶感染大鼠神经细胞在体外和体内。β-半乳糖苷酶在培养的几乎所有交感神经元和星形胶质细胞中表达。 在立体定向接种到大鼠海马和黑质后，检测β-半乳糖苷酶活性2个月。感染的细胞被确定为小胶质细胞，星形胶质细胞，或神经元的解剖，形态学和免疫组化标准。未观察到明显的细胞病变。
The efficient introduction of genetic material into quiescent nerve cells is important in the study of brain function and for gene therapy of neurological disorders. A replication-deficient adenoviral vector that contained a reporter gene encoding beta-galactosidase infected rat nerve cells in vitro and in vivo. Beta-galactosidase was expressed in almost all sympathetic neurons and astrocytes in culture. After stereotactic inoculations into the rat hippocampus and the substantia nigra, beta-galactosidase activity was detected for 2 months. Infected cells were identified as microglial cells, astrocytes, or neurons with anatomical, morphological, and immunohistochemical criteria. No obvious cytopathic effect was observed.