INTERACTION OF ALAMETHICIN WITH LECITHIN BILAYERS - A P-31 AND H-2 NMR-STUDY
INTERACTION OF ALAMETHICIN WITH LECITHIN BILAYERS - A P-31 AND H-2 NMR-STUDY
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DOI：
10.1021/bi00347a019
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发表时间：
1985-12-17
期刊：
影响因子：
2.9
通讯作者：
CHAN, SI
中科院分区：
文献类型：
--
作者：
BANERJEE, U;ZIDOVETZKI, R;CHAN, SI
采用核磁共振（NMR）和拉曼光谱（Raman）研究了阿拉梅蛋白与人工卵磷脂多层分散体的相互作用。核磁共振31P研究显示，在二肽的存在下，脂质头基团受到扰动。对31P核磁共振谱的模拟表明，所观察到的光谱变化可归因于头部基团的平均倾斜角的微小变化。相比之下，通过2H NMR和拉曼光谱测量，没有检测到肽对脂质分子疏水酰基链的段序的明显影响。综上所述，这些结果表明，在没有跨膜电位的情况下，alamethicin主要在水-脂质界面相互作用，而没有明显的插入或并入双分子层小叶。
The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the observed spectral changes could be attributed to slight variations in the average tilt angle of the head groups. In contrast, no noticeable effect of the peptide on the segmental order of the hydrophobic acyl chains of the lipid molecules was detected by 2H NMR and Raman spectroscopic measurements. Taken together, these results indicated that, in the absence of a transmembrane electric potential, alamethicin interacts primarily at the water-lipid interface without significant insertion or incorporation into the bilayer leaflet.