Immune response to acute otitis media in children III. Implications of viral antibody in middle ear fluid.
Immune response to acute otitis media in children III. Implications of viral antibody in middle ear fluid.
复制标题
儿童急性中耳炎的免疫反应Ⅲ．
作者：
J. Sloyer;V. Howie;J. H. Ploussard;J. Bradac;M. Habercorn;P. Ogra
应用放射免疫扩散和间接FA染色技术，检测了103例中耳炎患者血清和中耳液中麻疹、腮腺炎、风疹和脊髓灰质炎的IgA抗体。同时抽取的血清中有无IgA病毒抗体作为局部抗体产生的指标。在进行的401次检测中，发现41例MEF中有特异性IgA抗体。其中只有4例出现在未免疫患者的样本中，是针对风疹病毒的。在其余37例病例中，分别有16例针对麻疹和1号脊髓灰质炎，4例和1例分别针对腮腺炎和风疹病毒。免疫个体MEF的平均特异性IgA滴度比未免疫个体高8-17倍，并在免疫后至少持续9-19个月。结果表明：1)肠外免疫和口服免疫均可实现中耳粘膜的特异性免疫增敏；2)中耳粘膜存在特异性免疫记忆。这两个因素对急性中耳炎免疫的可行性至关重要。
With the technique of radioimmunodiffusion and indirect FA staining, IgA antibody to measles, mumps, rubella, and polio-1 were determined in serum and middle ear fluid (MEF) of 103 patients with otitis media. The occurrence of IgA viral antibody in MEF and its absence in simultaneously drawn serum was used as an indicator of local antibody production. Of the 401 assays performed, 41 instances of IgA antibody exclusively in MEF were found. Only four of these occurred in specimens from unimmunized patients and were directed against rubella virus. Of the 37 remaining cases, 16 each were directed against measles and polio-1 and four and one, respectively, for mumps and rubella viruses. The mean specific IgA titers were from 8- to 17-fold higher in MEF from immunized individuals than in the unimmunized and persisted for at least 9 to 19 months after immunization. The data thus indicate 1) specific immunologic sensitization of the middle ear mucosa can be achieved by parenteral as well as oral routes of immunization, and 2) specific immunologic memory exists in the middle ear mucosa. These two factors are crucial to feasibility of immunization against acute otitis media.