Quantitative in situ hybridization to measure single-cell changes in vasopressin and oxytocin mRNA levels after osmotic stimulation.
Quantitative in situ hybridization to measure single-cell changes in vasopressin and oxytocin mRNA levels after osmotic stimulation.
复制标题
定量原位杂交可测量渗透刺激后单细胞加压素和催产素 mRNA 水平的变化。
DOI：
10.1007/bf00733636
复制
发表时间：
1990
影响因子：
4
通讯作者：
Desharnais,RA
中科院分区：
文献类型：
--
作者：
McCabe,JT;Kawata,M;Sano,Y;Pfaff,DW;Desharnais,RA
1.由于脑组织具有高度的表型异质性，因此，应用原位杂交技术定量测定细胞mRNA含量是研究脑组织基因表达的一种有价值的方法。2.大鼠下丘脑-神经垂体系统神经元中加压素和催产素mRNA的含量在2%氯化钠溶液中维持24小时后发生变化，3.然后使用统计和图形技术逐个细胞地分析mRNA水平如何由于渗透刺激而改变。我们建议负二项概率分布是一个合适的模型来描述mRNA含量如何在一个定义的细胞群中变化。对于催产素和加压素mRNA水平的测量，最大似然估计表明，该模型充分描述了从饮用自来水或盐水的大鼠获得的实证研究结果。4.图形和统计分析表明，定义的神经系统如何响应渗透刺激：mRNA含量的改变作为一个乘法函数的“初始状态”。定量方法的效用和局限性进行了讨论。
1.The measurement of cellular mRNA content by quantitativein situhybridization is a valuable approach to the study of gene expression in brain since this tissue exhibits a high degree of phenotypic heterogeneity.2.The cellular content of vasopressin and oxytocin mRNA in hypothalamo-neurohypophysial system neurons was altered by maintaining rats for 24 hr on 2% sodium chloride water.3.Statistical and graphical techniques were then used to analyze cell by cell how mRNA levels were altered as a result of osmotic stimulation. We propose that the negative binomial probability distribution is a suitable model to describe how mRNA content varies across a defined cell population. For both measures of oxytocin and vasopressin mRNA levels, maximum-likelihood estimation indicated that this model adequately described empirical findings obtained from rats drinking tap water or salt water.4.Both graphical and statistical analyses suggested how the defined neural system responds to osmotic stimulation: mRNA content was altered as a multiplicative function of “initial state.” The utility and limitations of the quantitative approach are discussed.