One-dimensional SDS gel electrophoresis of proteins.
One-dimensional SDS gel electrophoresis of proteins.
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DOI：
10.1002/0471142727.mb1002as97
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Gallagher, Sean R
中科院分区：
文献类型：
--
作者：
Gallagher, Sean R
蛋白质的一维凝胶电泳提供了有关蛋白质样品的分子大小、数量和纯度的信息。分离的蛋白质可以从聚丙烯酰胺凝胶中回收，用于随后通过多种二级技术进行表征，例如质谱法以确定翻译后修饰和氨基酸序列。此外，一维电泳是免疫印迹和免疫检测的标准第一步。垂直平板凝胶中的蛋白质分离以多种形式进行。最近，小幅面微型凝胶由于其易于使用、相对低的成本和现成的商业可用性而成为典型。更大的凝胶提供了更大的分离面积，从而为复杂样品提供了更好的分辨率，并继续用于专业分析。
One-dimensional gel electrophoresis of proteins provides information about the molecular size, amount, and purity of a protein sample. Separated proteins can be recovered from polyacrylamide gels for subsequent characterization by a variety of secondary techniques, such as mass spectrometry to determine post-translational modifications and the amino acid sequence. In addition, one-dimensional electrophoresis is the standard first step in immunoblotting and immunodetection. Protein separations in vertical slab gels are performed in a variety of formats. Most recently, small format minigels are typical due to their ease of use, low relative cost, and ready commercial availability. Larger gels provide more separation area and thus better resolution for complex samples and continue to be in use for specialized analysis.