Fluorescence, reflectance, and light-scattering spectroscopy for evaluating dysplasia in patients with Barrett's esophagus
Fluorescence, reflectance, and light-scattering spectroscopy for evaluating dysplasia in patients with Barrett's esophagus
复制标题
DOI：
10.1053/gast.2001.24842
复制
发表时间：
2001-06-01
期刊：
影响因子：
29.4
通讯作者：
Feld, MS
中科院分区：
文献类型：
--
作者：
Georgakoudi, I;Jacobson, BC;Feld, MS
（背景和目的）在bar下：本研究的目的是评估3种光谱技术（荧光、反射和光散射光谱）单独和组合用于评估Barrett食管（BE）患者低度和高度异型增生的潜力。使用光纤探针在活检前约1秒内从每个部位采集11个激发波长的荧光光谱和反射光谱。测量的荧光光谱与反射光谱相结合，以提取内在组织荧光。反射光谱提供了大量组织的形态学信息，而光散射光谱被用来确定细胞核拥挤和扩大巴雷特上皮。（结果）bar下：发育异常和非发育异常BE之间的内在荧光，体散射特性，上皮细胞核拥挤和扩大的水平方面观察到显着差异。所有3种技术的组合导致区分高级别与非高级别以及异型增生与非异型增生上皮的上级敏感性和特异性。（结论）在酒吧之下：内源性荧光、反射和光散射光谱提供了有关发育异常发生过程中发生的生化和形态学变化的补充信息，这些技术（三模态光谱）的组合可以作为评价BE发育异常的极好工具。
(Background & Aims) under bar: The aim of this study was to assess the potential of 3 spectroscopic techniques (fluorescence, reflectance, and light-scattering spectroscopy) individually and in combination, for evaluating low- and high-grade dysplasia In patients with Barrett's esophagus (BE), (Methods) under bar: Fluorescence spectra at 11 excitation wavelengths and a reflectance spectrum were acquired in approximately 1 second from each site before biopsy using an optical fiber probe. The measured fluorescence spectra were combined with the reflectance spectra to extract the intrinsic tissue fluorescence. The reflectance spectra provided morphologic information about the bulk tissue, whereas light-scattering spectroscopy was used to determine cell nuclear crowding and enlargement in Barrett's epithelium. (Results) under bar: Significant differences were observed between dysplastic and non-dysplastic BE in terms of intrinsic fluorescence, bulk scattering properties, and levels of epithelial cell nuclear crowding and enlargement. The combination of all 3 techniques resulted In superior sensitivity and specificity for separating high-grade from non-high-grade and dysplastic from nondysplastic epithelium. (Conclusions) under bar: Intrinsic fluorescence, reflectance, and light-scattering spectroscopies provide complementary information about biochemical and morphologic changes that occur during the development of dysplasia, The combination of these techniques (Tri-Modal Spectroscopy) can serve as an excellent tool for the evaluation of dysplasia in BE.