Effect of Different Selenium Supplementation Levels on Oxidative Stress, Cytokines, and Immunotoxicity in Chicken Thymus
Effect of Different Selenium Supplementation Levels on Oxidative Stress, Cytokines, and Immunotoxicity in Chicken Thymus
复制标题
DOI：
10.1007/s12011-015-0598-7
复制
发表时间：
2016-01
影响因子：
3.9
通讯作者：
Yachao Wang;Li Jiang;Yuanfeng Li;Xuegang Luo;Jian He
中科院分区：
文献类型：
--
作者：
Yachao Wang;Li Jiang;Yuanfeng Li;Xuegang Luo;Jian He
研究了不同硒水平对鸡胸腺氧化应激、细胞因子和免疫毒性的影响。将180只1日龄产蛋鸡随机分为4组（n= 45）。试验鸡分别饲喂含硒0.2mg/kg的基础日粮（对照组）、含硒5 mg/kg的低补日粮、含硒10 mg/kg的中补日粮和含硒15 mg/kg的高补日粮15、30和45 d。在整个实验期间，收集血清和胸腺样品并用于检测实验指数。结果表明，除15日龄补硒5 mg/kg组外，高硒组各时间点胸腺抗氧化酶活性、抗氧化酶IFN-γ和IL-2 mRNA水平及血清IFN-γ和IL-2含量均显著低于对照组（P< 0.05）。与对照组相比，补硒5 mg/kg组血清和胸腺中IFN-γ的含量在15和30日龄时显著升高（P< 0.05）。病理组织学检查发现，高硒组鸡胸腺组织出现不同程度的皮质下降、髓质增厚、网状细胞变性。结果提示，过量硒可导致机体免疫功能下降，氧化损伤加重，并出现皮质下降、髓质增厚、网状细胞变性等一系列临床病理改变。
This study assessed the effects of different selenium (Se) supplementation levels on oxidative stress, cytokines, and immunotoxicity in chicken thymus. A total of 180 laying hens (1 day old; Mianyang, China) were randomly divided into 4 groups (n= 45). The chickens were maintained either on a basic diet (control group) containing 0.2 mg/kg Se, a low-supplemented diet containing 5 mg/kg Se, a medium-supplemented diet containing 10 mg/kg Se, or a high-supplemented diet containing 15 mg/kg Se for 15, 30, and 45 days, respectively. Over the entire experimental period, serum and thymus samples were collected and used for the detection of the experimental index. The results indicated that the antioxidative enzyme activities and messenger RNA (mRNA) levels of antioxidative enzymes, IFN-γ and IL-2 in the thymus, and the content of IFN-γ and IL-2 in the serum of excessive-Se-treated chickens at all time points (except for the 5 mg/kg Se supplement group at 15 days) were significantly decreased (P< 0.05) compared to the corresponding control groups. Interestingly, a significantly increase (P< 0.05) in the content of IFN-γ was observed in the serum and thymus in the 5 mg/kg Se supplement group at 15 and 30 days compared to the corresponding control groups. In histopathological examination, the thymus tissue from excessive-Se-treated chickens revealed different degrees of cortex drop, incrassation of the medulla, and degeneration of the reticular cells. These results suggested that the excessive Se could result in a decrease in immunity, an increase in oxidative damage, and a series of clinical pathology changes, such as cortex drop, incrassation of the medulla, and degeneration of the reticular cells.