Identification of cyanobacterial cell division genes by comparative and mutational analyses
Identification of cyanobacterial cell division genes by comparative and mutational analyses
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DOI：
10.1111/j.1365-2958.2005.04548.x
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发表时间：
2005-04-01
影响因子：
3.6
通讯作者：
Osteryoung, KW
中科院分区：
文献类型：
--
作者：
Miyagishima, SY;Wolk, CP;Osteryoung, KW
我们进行了比较和突变分析，以更全面地定义参与蓝藻细胞分裂的基因库。基因ftsE，ftsI，ftsQ，ftsW，和（以前公认的）ftsZ，minC，minD，minE和苏拉被确定为革兰氏阴性和革兰氏阳性细菌的细胞分裂基因的同源物。细长聚球藻PCC 7942的转座子诱变鉴定了另外五个参与细胞分裂的基因，cdv1、cdv2、cdv3、ftn6和cikA。CDV 1编码假定的周质肽基脯氨酰顺反异构酶。cdv 2与ylmF具有相似性，ylmF与divIVA一样位于革兰氏阳性细菌ylm基因簇内，其成员具有与分裂相关的功能。蓝细菌中其他ylm基因的保守性表明蓝细菌和革兰氏阳性菌共享特定的分裂蛋白。两个ylm同源物也在藻类和植物基因组中发现。cdv3与divIVA具有低但显著的相似性，表明minE和cdv3都介导蓝藻中的分裂位点确定。相反，革兰氏阳性细菌缺乏minE，（革兰氏阴性）变形菌缺乏divIVA。ftn6，未知的功能，和昼夜节律输入激酶，cikA，是特定的蓝藻。In S.与其他细菌不同，FtsZ环在被类核占据的位点形成。因此，蓝细菌的分裂机制在其组成和调节方面不同于革兰氏阴性和革兰氏阳性细菌。
We performed comparative and mutational analyses to define more comprehensively the repertoire of genes involved in cyanobacterial cell division. Genes ftsE, ftsI, ftsQ, ftsW, and (previously recognized) ftsZ, minC, minD, minE and sulA were identified as homologues of cell division genes of Gram-negative and Gram-positive bacteria. Transposon mutagenesis of Synechococcus elongatus PCC 7942 identified five additional genes, cdv1, cdv2, cdv3, ftn6 and cikA, involved in cell division. cdv1 encodes a presumptive periplasmic peptidyl-prolyl cis-trans isomerase. cdv2 has similarity to ylmF which, like divIVA, lies within the Gram-positive bacterial ylm gene cluster whose members have functions associated with division. Conservation of other ylm genes in cyanobacteria suggests that cyanobacteria and Gram-positive bacteria share specific division proteins. Two ylm homologues are also found in algal and plant genomes. cdv3 has low but significant similarity to divIVA, suggesting that minE and cdv3 both mediate division-site determination in cyanobacteria. In contrast, Gram-positive bacteria lack minE, and (Gram-negative) proteobacteria lack divIVA. ftn6, of unknown function, and the circadian input kinase, cikA, are specific to cyanobacteria. In S. elongatus, unlike in other bacteria, FtsZ rings are formed at sites occupied by nucleoids. Thus, the division machinery of cyanobacteria differs in its composition and regulation from that of Gram-negative and Gram-positive bacteria.