Acidic polysaccharide isolated from Phellinus linteus enhances through the up-regulation of nitric oxide and tumor necrosis factor-α from peritoneal macrophages
Acidic polysaccharide isolated from Phellinus linteus enhances through the up-regulation of nitric oxide and tumor necrosis factor-α from peritoneal macrophages
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DOI：
10.1016/j.jep.2004.06.024
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发表时间：
2004-11-01
影响因子：
5.4
通讯作者：
Park, YM
中科院分区：
文献类型：
--
作者：
Kim, GY;Choi, GS;Park, YM
药用蘑菇越来越多地被用于治疗各种疾病过程。桑黄子实体或菌丝体的水提物在体内和体外都具有抗肿瘤和免疫调节活性。然而，其杀瘤作用的潜在机制却知之甚少。桑黄酸性多糖组分对B16黑色素瘤细胞的杀瘤活性呈剂量依赖性增强，200µg/ml的酸性多糖组对黑色素瘤细胞的生长抑制率增加4倍。为了进一步研究PL的作用机制，我们研究了PL对巨噬细胞吞噬功能和一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)和活性氧中间体(ROI)释放的影响。为了研究PM的吞噬功能，比较了未处理和PL处理的PM对葡聚糖(Dex)-FITC的摄取。我们发现，与未处理组相比，经PL处理的PM的吞噬功能有所增强。白三烯可剂量依赖性地刺激PM大鼠血清中NO和TNF-α水平升高，但对ROI的产生无明显影响我们认为PL对Yac-1细胞具有细胞毒作用，其机制可能是通过上调NO和TNF-α的产生来实现的。此外，PL还增强了PM中共刺激分子CD80和CD86以及主要组织相容性复合体(MHC)分子II的表达通过推测，PL上调这些参与抗原呈递过程的表面分子的能力，可能会激活体内T细胞介导的针对恶性细胞的免疫。综上所述，这些结果表明PL是一种有效的免疫调节剂，并增强了PM的抗肿瘤活性(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
Medicinal mushrooms are increasingly used to treat a wide variety of disease processes. Aqueous extract from the fruiting body or mycelia of Phellinus linteus has been reported to produce antitumor and immunomodulatory activities in vivo and in vitro. However, the mechanisms underlying its tumoricidal effects are poorly understood. The tumoricidal activity of peritoneal macrophages (PM) cultured with acidic polysaccharide (PL) isolated from Phellinus linteus against B16 melanoma cells was enhanced in a dose-dependent manner; growth inhibition increased 4-fold with 200 mug/ml of PL. To further characterize the mechanisms of PL, we investigated the effects of PL on phagocytosis and the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha) and reactive oxygen intermediates (ROI). To investigate the phagocytosis of PM, the uptake of Dextran (Dex)-FITC between PL-untreated and PL-treated PM was compared. We found some augment in phagocytosis of PL-treated PM compared untreated group. PL stimulated a dose-dependent increase in NO and TNF-a, but not in ROI production in PM. We suggested that PL has cytotoxicity against Yac-1 cells through the up-regulation of NO and TNF-a production. Also, PL enhanced the expression of costimulatory molecules, CD80 and CD86, and major histocompatibility complex (MHC) molecules II in PM. The ability of PL upon the up-regulation of these surface molecules involved in antigen-presenting processes may, by inference, activate T-cell-mediated immunity against malignant cells in vivo. Taken together, these results suggest that PL act as an effective immunomodulator and enhances the anti-tumoral activity of PM. (C) 2004 Elsevier Ireland Ltd. All rights reserved.