Immunologic enhancement of compound Chinese herbal medicinal ingredients and their efficacy comparison with compound Chinese herbal medicines.
Immunologic enhancement of compound Chinese herbal medicinal ingredients and their efficacy comparison with compound Chinese herbal medicines.
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DOI：
10.1016/j.vaccine.2005.11.053
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发表时间：
2006-03
期刊：
影响因子：
5.5
通讯作者：
Junling Sun;Yuanliang Hu;Deyun Wang;Baokang Zhang;Jiaguo Liu
中科院分区：
文献类型：
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作者：
Junling Sun;Yuanliang Hu;Deyun Wang;Baokang Zhang;Jiaguo Liu
以淫羊藿多糖（EPS）和黄芪多糖（APS）为原料，分别与淫羊藿总黄酮（PF）和黄芪总黄酮（GS）配伍，制备了两种中药复方制剂。以淫羊藿、黄芪、人参为原料，制备了两种成分含量与相应的复方中药制剂相同的复方中药制剂。在家兔免疫试验中，将两种cCI分别注射给兔出血症灭活疫苗免疫的家兔，对照组为生理盐水。于接种后第3、7、10、14、21、28、35、42天，采用血凝抑制（HI）试验测定血清抗体滴度的动态变化。在鸡免疫试验中，将所有cCI和cCM分别与灭活的纽卡斯尔疫苗病毒混合免疫鸡，以油佐剂和非佐剂疫苗作为对照。分别于免疫后第7、14、21、28、35、42天，用MTT法和HI法检测外周血淋巴细胞增殖和血清抗体滴度的动态变化。结果表明，两种cCI均能显著提高家兔抗体效价，其中复方中药成分1（cCI 1）的效果优于复方中药成分2（cCI 2）。cCIs和cCMs均能显著促进鸡淋巴细胞增殖和提高抗体效价，与油佐剂相似，cCIs的免疫增强作用略优于cCMs的免疫增强作用上级。
Two compound Chinese herbal medicinal ingredients (cCIs) were prepared respectively with epimedium polysaccharide (EPS) plus propolis flavone (PF) and astragalus polysaccharide (APS) plus ginsenoside (GS). Also, two compound Chinese herbal medicines (cCMs) with the same ingredient content as corresponding cCIs were made with the extracts of epimedium plus propolis and astragalus plus ginseng. In rabbit immune trial, two cCIs, physiological saline in the control, were respectively injected to the rabbits vaccinated with inactivated rabbit hemorrhagic disease vaccine. On Days 3, 7, 10, 14, 21, 28, 35 and 42 after vaccination, the dynamic changes of serum antibody titers were determined by hemagglutination inhibition (HI) test. In chicken immune trial, all of cCIs and cCMs were mixed respectively with inactivated Newcastle disease vaccine virus to vaccinate chickens, taking oil-adjuvant and non-adjuvant vaccine as controls. On Days 7, 14, 21, 28, 35 and 42 after vaccination, the dynamic changes of peripheral lymphocyte proliferation and serum antibody titers were tested respectively by MTT method and HI test method. The results showed that both cCIs could significantly raise antibody titer in rabbits, which the effect of compound Chinese herbal medicinal ingredients 1 (cCIs 1) was better than that of compound Chinese herbal medicinal ingredients 2 (cCIs 2). All of cCIs and cCMs could markedly promote lymphocyte proliferation and enhance antibody titer in chickens, which was similar to oil adjuvant, the immunologic enhancement of cCIs were slightly superior to that of the cCMs.