Intrapleural activation, processing, efficacy, and duration of protection of single-chain urokinase in evolving tetracycline-induced pleural injury in rabbits.
Intrapleural activation, processing, efficacy, and duration of protection of single-chain urokinase in evolving tetracycline-induced pleural injury in rabbits.
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单链尿激酶在兔四环素诱导的胸膜损伤中的胸膜内激活、处理、功效和保护持续时间。
DOI：
10.1152/ajplung.00118.2006
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Azghani,Ali
中科院分区：
文献类型：
--
作者：
Idell,Steven;Allen,Timothy;Chen,Shande;Koenig,Kathy;Mazar,Andrew;Azghani,Ali
Intrapleural fibrinolysins have been used to treat pleural loculations. However, the efficacy of clinically available agents has recently been questioned, providing a rationale for investigation of new interventions. Single-chain urokinase plasminogen activator resists inhibition by serpins, and repeated, daily intrapleural administration of this agent prevents intrapleural loculation more effectively than complexes of this proenzyme with its receptor (Idell S, Mazar A, Cines D, Kuo A, Parry G, Gawlak S, Juarez J, Koenig K, Azghani A, Hadden W, McLarty J, Miller E.Am J Respir Crit Care Med166: 920–926, 2002). Understanding of the protective mechanism and intrapleural processing remains unclear. We speculated that single-chain urokinase could induce sustained local fibrinolysis and protection by selective administration either before, during, or following loculation after pleural injury induced by tetracycline in rabbits. Enzymography, immunoassays, histology, immunohistochemistry, morphology, and morphometry were used to test the efficacy, duration of protective effect, and processing of single-chain urokinase. Intrapleural single chain urokinase prevented loculation at 72 h after injury (P< 0.01) if given either before or during adhesion formation and was converted to two-chain high-molecular-weight urokinase, which remained active for at least 24 h within pleural fluids. The effect was dose dependent, and established loculations at 72 h after tetracycline-induced injury were reversed at 96 h by single-dose treatment. Single-chain urokinase bound and saturated intrapleural plasminogen activator inhibitory (PAI)-1-like activity and urokinase-related immunoreactivity of the mesothelium was comparable in treatment or vehicle-control groups. Adhesions recurred by 2 wk after treatment with recurrence of excess local PAI activity. Single-chain urokinase induces sustained local fibrinolysis and reversibly prevents pleural loculation for up to 48 h after intrapleural administration after tetracycline-induced injury.
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影响因子：
158.5
作者：
Maskell, NA;Davies, CWH;Davies, RJO
通讯作者：
Davies, RJO
影响因子：
24.3
作者：
Bouros, D;Schiza, S;Siafakas, N
通讯作者：
Siafakas, N
DOI：
10.1164/rccm.200204-313oc
发表时间：
2002-10-01
影响因子：
24.7
作者：
Idell, S;Mazar, A;Miller, E
通讯作者：
Miller, E
这么多年过去了，脓胸治疗的多中心试验。
DOI：
10.1056/nejme048352
发表时间：
2005
期刊：
The New England journal of medicine
影响因子：
--
作者：
J. Heffner
通讯作者：
J. Heffner
链激酶-链道酶在治疗肺炎后脓胸中的应用。
DOI：
10.1016/s0096-5588(20)31273-3
发表时间：
1951
期刊：
The Journal of thoracic surgery
影响因子：
--
作者：
W. Tillett;S. Sherry;Read Ct
通讯作者：
Read Ct