Protective Effect of Salvia Przewalskii Extract on Puromycin-Induced Podocyte Injury
Protective Effect of Salvia Przewalskii Extract on Puromycin-Induced Podocyte Injury
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DOI：
10.1159/000440851
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发表时间：
2015-01-01
影响因子：
4.2
通讯作者：
Ren, Hong-qi
中科院分区：
文献类型：
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作者：
Dai, De-shu;Liu, Xiang;Ren, Hong-qi
背景资料：研究甘西鼠尾草总酚酸提取物（SPE）对嘌呤霉素氨基糖苷（PAN）诱导的大鼠足细胞损伤的影响。方法：将大鼠分为PAN组、PAN+他克莫司组和SPE组。我们评估了SPE对足细胞损伤的影响，治疗后5，10，15和21天。结果：（1）各组大鼠均从第天开始出现蛋白尿。他克莫司组和高剂量SPE组的蛋白尿峰值水平不同，相对于PAN组和低、中剂量SPE组，其蛋白尿显著降低。各组的蛋白尿在第15天减少，并在第21天恢复到正常水平。(2)H&E和PAS染色显示肾小球形态无异常。电镜观察发现，从第5天开始，各组大鼠均出现足突消失，但他克莫司组和高剂量SPE组大鼠的足突消失程度较低。(3)nephrin和podocin的IHC染色显示高剂量SPE和他克莫司组的表达未受影响，线性分布更好。Western blot分析证实，SPE能促进蛋白质的表达。(4)他克莫司组和SPE高剂量组nephrin和podocin的mRNA水平显著高于其他各组。结论：在我们的研究中，我们首先证明了SPE能够减少蛋白尿，保护足细胞的形态和结构，并保持PAN诱导的大鼠足细胞损伤的裂膜蛋白水平。(C)2015 S. Karger AG，巴塞尔
Background: To determine the effect of Salvia przewalskii extract (SPE) from total phenolic acids on puromycin aminonucleoside (PAN)-induced rat podocyte injury. Methods: The rats were divided into groups that were treated with either PAN only or PAN followed by tacrolimus or SPE. We evaluated the effects of SPE on podocyte injury 5, 10, 15 and 21 days following treatment. Results: (1) Proteinuria was observed starting on days in all groups. The peak levels of proteinuria differed among the groups with tacrolimus and high-dose SPE, which significantly decreased proteinuria relative to the PAN and low- and medium-dose SPE groups. The proteinuria in each group decreased by day 15 and returned to a normal level by day 21. (2) H&E and PAS staining revealed no abnormality in glomerular morphology. With electron microscopy, we observed foot process effacement in the rats of all groups starting on day 5, but rats in the tacrolimus and high-dose SPE groups exhibited a lower degree. (3) IHC staining of nephrin and podocin revealed unaffected expression and better linear distributions in the high-dose SPE and tacrolimus groups. Western blot analysis confirmed that SPE could improve the expression of proteins. (4) The mRNA levels of nephrin and podocin in the tacrolimus and high-dose SPE groups were significantly higher than that in the others. Conclusion: In our study, we first demonstrated the ability of SPE to reduce proteinuria, preserve the morphology and structure of podocytes and retain the levels of slit diaphragm proteins on PAN-induced rat podocytes injury. (C) 2015 S. Karger AG, Basel