Transformation of the mushroom species Hypsizigus marmoreus, Flammulina velutipes, and Grifola frondosa by an Agrobacterium-mediated method using a universal transformation plasmid
Transformation of the mushroom species Hypsizigus marmoreus, Flammulina velutipes, and Grifola frondosa by an Agrobacterium-mediated method using a universal transformation plasmid
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使用通用转化质粒通过农杆菌介导方法转化蘑菇品种真姬菇、金针菇和灰树花
DOI：
10.1016/j.myc.2012.08.002
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发表时间：
2013
期刊：
影响因子：
1.4
通讯作者：
C. Tanaka
中科院分区：
文献类型：
--
作者：
K. Hatoh;K. Izumitsu;A. Morita;K. Shimizu;A. Ohta;M. Kawai;T. Yamanaka;H. Neda;Y. Ota;C. Tanaka
采用根癌农杆菌介导法（Agrobacterium tumefaciens，AMT）对真姬菇、金针菇和灰树花3种重要经济真菌的菌丝体进行了转化。我们使用了潮霉素B抗性基因（hph）的控制下的新型隐球菌肌动蛋白启动子。86株H. marmoreus、F. velutipes和G. frondosa获得。所有转化子均对潮霉素B具有高度抗性，表明C. neoformans actin启动子在担子菌中具有潜在的通用启动子活性。Southern分析显示hph基因的随机但单一整合。© 2012日本真菌学会。农杆菌介导的植物遗传转化（Agrobacterium tumefaciens mediated transformation，AMT）已被广泛应用于多种植物的遗传工程。自Bundock et al.（1995）通过使用AMT成功地转化了酿酒酵母（Saccharomyces cerevisiae），该方法已用于真菌（De Groot等，1998）。已经报道了在各种重要的真菌中通过AMT的转化，包括蘑菇物种双孢蘑菇（Agaricus bisporus）（Chen等人，2000），
Agrobacterium tumefaciens-mediated transformation (AMT) was successfully applied to mycelia of the 3 economically important mushrooms Hypsizigus marmoreus, Flammulina velutipes, and Grifola frondosa. We used the hygromycin B resistance gene (hph) under the control of the Cryptococcus neoformans actin promoter. Eighty-six resistant strains of H. marmoreus, 4 of F. velutipes, and 2 of G. frondosa were obtained. All transformants were highly resistant to hygromycin B, suggesting that the C. neoformans actin promoter has a potential universal promoter activity in basidiomycetes. Southern analysis revealed random but single integration of the hph gene.© 2012 The Mycological Society of Japan. Published by Elsevier BV All rights reserved.Agrobacterium tumefaciens-mediated transformation (AMT) has been used for genetic engineering of variety of plants. Since Bundock et al.(1995) succeeded in transformation of Saccharomyces cerevisiae by using AMT, this method has come to be used for fungi (De Groot et al. 1998). Transformation by AMT has been reported in various important fungi, including the mushroom species Agaricus bisporus (Chen et al. 2000),