Colon cancer-specific diagnostic and prognostic biomarkers based on genome-wide abnormal DNA methylation.
Colon cancer-specific diagnostic and prognostic biomarkers based on genome-wide abnormal DNA methylation.
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基于全基因组异常 DNA 甲基化的结肠癌特异性诊断和预后生物标志物
DOI：
10.18632/aging.103874
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发表时间：
2020-11-17
期刊：
影响因子：
--
通讯作者：
Wei M
中科院分区：
文献类型：
--
作者：
Wang Y;Zhang M;Hu X;Qin W;Wu H;Wei M
Abnormal DNA methylation is a major early contributor to colon cancer (COAD) development. We conducted a cohort-based systematic investigation of genome-wide DNA methylation using 299 COAD and 38 normal tissue samples from TCGA. Through conditional screening and machine learning with a training cohort, we identified one hypomethylated and nine hypermethylated differentially methylated CpG sites as potential diagnostic biomarkers, and used them to construct a COAD-specific diagnostic model. Unlike previous models, our model precisely distinguished COAD from nine other cancer types (e.g., breast cancer and liver cancer; error rate ≤ 0.05) and from normal tissues in the training cohort (AUC = 1). The diagnostic model was verified using a validation cohort from The Cancer Genome Atlas (AUC = 1) and five independent cohorts from the Gene Expression Omnibus (AUC ≥ 0.951). Using Cox regression analyses, we established a prognostic model based on six CpG sites in the training cohort, and verified the model in the validation cohort. The prognostic model sensitively predicted patients’ survival (p ≤ 0.00011, AUC ≥ 0.792) independently of important clinicopathological characteristics of COAD (e.g., gender and age). Thus, our DNA methylation analysis provided precise biomarkers and models for the early diagnosis and prognostic evaluation of COAD.
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影响因子：
5.7
作者：
Lind GE;Guriby M;Ahlquist T;Hussain I;Jeanmougin M;Søreide K;Kørner H;Lothe RA;Nordgård O
通讯作者：
Nordgård O
DOI：
10.1158/1055-9965.epi-13-0143
发表时间：
2013-06
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Andaya AA;Enewold L;Zahm SH;Shriver CD;Stojadinovic A;McGlynn KA;Zhu K
通讯作者：
Zhu K
影响因子：
3.7
作者：
Islam, Farhadul;Gopalan, Vinod;Lam, Alfred King-yin
通讯作者：
Lam, Alfred King-yin
影响因子：
14.9
作者：
Franceschini A;Szklarczyk D;Frankild S;Kuhn M;Simonovic M;Roth A;Lin J;Minguez P;Bork P;von Mering C;Jensen LJ
通讯作者：
Jensen LJ
影响因子：
7.5
作者：
Ma, Changqing;Olevian, Dane;Pai, Reetesh K.
通讯作者：
Pai, Reetesh K.