Identification and functional analysis of new conserved microRNAs and their targets in potato (Solanum tuberosum L.)
Identification and functional analysis of new conserved microRNAs and their targets in potato (Solanum tuberosum L.)
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DOI：
10.3906/bot-1405-105
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发表时间：
2014-01-01
影响因子：
1.8
通讯作者：
Baloch, Iftekhar Ahmed
中科院分区：
文献类型：
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作者：
Din, Muhammad;Barozai, Muhammad Younas Khan;Baloch, Iftekhar Ahmed
微小RNA（microRNAs，miRNAs）是一种微小的内源性非蛋白质编码负调控因子，参与转录后基因的调控。马铃薯（Solanum tuberosum L.）是一种淀粉和块茎作物，其钾和维生素含量也很重要。虽然马铃薯是世界上第四大粮食作物，但其miRNAs及其靶点尚未得到很好的研究。在这里，我们报告了一个比较基因组学的方法，适用于探索微RNA及其靶蛋白在马铃薯。从表达的序列标签中鉴定和表征了来自110个家族的共120个新的miRNA。观察到所有120种miRNA，沿着稳定的茎环前体结构，其长度范围为42至488 nt，平均为132 nt。成熟的miRNAs长度范围为18 ~ 26 nt，平均21 nt。随后，随机选择10个miRNAs，通过逆转录聚合酶链反应进行验证。预测了这120种新的马铃薯miRNAs共有433个潜在靶点。这些靶点参与调控、代谢、转录因子、生长发育等生理过程。这些miRNAs及其靶标将有助于微调马铃薯以获得更好的食物含量和更高的营养价值以及生物和非生物胁迫抗性。
MicroRNAs (miRNAs) are tiny, endogenous, nonprotein-coding, and functionally negative regulators of posttranscriptional gene regulation. Potato (Solanum tuberosum L.) is a starchy and tuberous crop that is also important for its potassium and vitamin contents. Though potato is the world's fourth largest food crop, its miRNAs and their targets have not been well studied. Here we report a comparative genomics approach that was applied to explore miRNAs and their targeted proteins in potato. A total of 120 new miRNAs from 110 families were identified and characterized from the expressed sequence tags. All 120 miRNAs were observed, along with stable stem-loop precursor structures, whose lengths ranged from 42 to 488 nt with an average of 132 nt. Mature miRNAs lengths ranged from 18 to 26 nt with an average of 21 nt. Later, 10 miRNAs were randomly selected for validation by reverse-transcription polymerase chain reaction. A total of 433 potential targets were predicted for these new 120 potato miRNAs. These targets were involved in regulation, metabolism, transcription factors, growth and development, and other physiological processes. These miRNAs and their targets will be useful to fine-tune potatoes for better food content and higher nutritional value as well biotic and abiotic stress resistance.