Myocardial osteopontin expression is associated with left ventricular hypertrophy
Myocardial osteopontin expression is associated with left ventricular hypertrophy
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DOI：
10.1161/01.cir.96.9.3063
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发表时间：
1997-11-04
期刊：
影响因子：
37.8
通讯作者：
Hsueh, WA
中科院分区：
文献类型：
--
作者：
Graf, K;Do, YS;Hsueh, WA
背景骨桥蛋白（OP）已被确定在培养的大鼠心脏成纤维细胞，在那里它有助于血管紧张素II（AII）诱导的重塑过程;在培养的心肌细胞;和巨噬细胞在心脏组织的炎症。然而，在心肌组织的组织学切片中尚未报道OP的存在。本实验研究了（1）培养的大鼠心肌细胞中OP mRNA表达的调控，（2）正常和肥厚的新生和成年大鼠心肌中OP mRNA的定位，以及OP mRNA在心肌细胞中的表达。以及（3）方法和结果培养的乳鼠心肌细胞表达OP mRNA，并与正常心肌细胞呈免疫反应性。内皮素-1（ET-1）和去甲肾上腺素（NE）使OP和心钠素（ANP）mRNA水平增加2 ~ 3倍（P
Background Osteopontin (OP) has been identified in cultured rat cardiac fibroblasts, where it contributes to angiotensin II (AII)-induced remodeling processes; in cultured cardiomyocytes; and in macrophages in cardiac tissues with inflammation. However, the presence of OP has not been reported in histological sections of myocardial tissue. In the present study, we investigated (1) the regulation of OP mRNA expression in cultured rat cardiomyocytes; (2) the localization of OP mRNA in neonatal and adult normal and hypertrophied rat hearts; and (3) the histology of OP expression in myocardial specimens from humans either with myocyte hypertrophy or with no pathological changes.Methods and Results Cultured neonatal cardiomyocytes expressed OP mRNA and were immunoreactive for OP. Endothelin-1 (ET-1) and norepinephrine (NE) increased both OP and atrial natriuretic peptide (ANP) mRNA levels twofold to threefold (P