FGF binding by extracellular matrix components of Wharton's jelly
FGF binding by extracellular matrix components of Wharton's jelly
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DOI：
10.18388/abp.2007_3257
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Bankowski, Edward
中科院分区：
文献类型：
--
作者：
Malkowski, Andrzej;Sobolewski, Krzysztof;Bankowski, Edward
我们早期的论文已经报道，华顿氏胶是几种肽生长因子的储库，包括酸性和碱性成纤维细胞生长因子（分别为aFGF和bFGF）。两者都可以通过缓冲盐溶液以高分子量复合物的形式提取，可能具有细胞外基质的组分。在电泳过程中，来自这种提取物的aFGF和bFGF几乎不能穿透10%聚丙烯酰胺凝胶。用透明质酸酶预处理华顿胶略微增加aFGF的可提取性，但不影响bFGF的可提取性。相反，用细菌胶原酶（2000 U/ ml，37 ℃，18 h）预处理组织匀浆增加bFGF的可提取性。提取溶液中β-巯基乙醇的存在增加了两种FGF的可提取性，但不释放游离形式的FGF，尽管降低了含FGF复合物的分子量。我们的结论是，aFGF和bFGF通过二硫键结合到沃顿氏胶的蛋白质成分。我们建议，主要由胶原纤维和透明质酸分子组成的基质，其包围着华顿氏胶细胞，防止提取液进入aFGF和bFGF。虽然透明质酸盐和胶原不直接结合aFGF或bFGF，但它们可构成阻止FGF在华顿氏胶中分散的屏障。因此，华顿氏胶质细胞周围的高浓度FGF可能促进这些因子与膜受体的相互作用，从而导致细胞分裂和分化的刺激，以及细胞外基质成分的合成。
Our earlier paper has reported that Wharton's jelly is a reservoir of several peptide growth factors, including acidic and basic fibroblast growth factors (aFGF and bFGF, respectively). Both can be extracted by buffered salts solutions in the form of high molecular mass complexes, probably with a component(s) of the extracellular matrix. Both aFGF and bFGF from such extracts hardly penetrate 10% polyacrylamide gels during electrophoresis. Pre-treatment of Wharton's jelly with hyaluronidase slightly increased the extractability of aFGF, but did not affect the extractability of bFGF. In contrast, the pre-treatment of tissue homogenate with bacterial collagenase (2000 U/ ml, 37 degrees C, 18 h) increased the extractability of bFGF. The presence of beta-mercaptoethanol in the extracting solutions increased the extractability of both FGFs, but did not release FGFs in their free form, despite reducing the molecular mass of the FGF-containing complexes. We conclude that both aFGF and bFGF are bound through disulphide bonds to a protein component of Wharton's jelly. We propose that ground substance composed mainly of collagen fibrils and hyaluronate molecules, which surrounds the cells of Wharton's jelly, prevents the access of the extracting solution to aFGF and bFGF. Although hyaluronate and collagen do not bind aFGF or bFGF directly, they may constitute a barrier which prevents the dispersion of FGFs in Wharton's jelly. Thus, the high concentration of FGFs around the cells of Wharton's jelly may facilitate the interaction of these factors with membrane receptors, thereby resulting in stimulation of cell division and differentiation, as well as of the synthesis of extracellular matrix components.