Hormonal regulation of meiotic maturation in the hamster oocyte involves a cytoskeleton-mediated process.
Hormonal regulation of meiotic maturation in the hamster oocyte involves a cytoskeleton-mediated process.
复制标题
仓鼠卵母细胞减数分裂成熟的激素调节涉及细胞骨架介导的过程。
DOI：
10.1095/biolreprod51.5.852
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发表时间：
1994
影响因子：
3.6
通讯作者：
Albertini,DF
中科院分区：
文献类型：
--
作者：
Plancha,CE;Albertini,DF
哺乳动物卵母细胞的成熟包括受精诱导的减数分裂细胞周期从前期I到中期II的进展和第一极体（PB）的排出。在这项研究中，促性腺激素对培养的仓鼠卵母细胞减数分裂细胞周期进程的影响进行了分析卵丘细胞-卵母细胞相互作用和卵母细胞细胞骨架的变化。卵丘-卵母细胞复合体从hCG致敏动物的大窦卵泡（直径700 μ m）中获得，并在单独促性腺激素（FSH、hCG、LH）或联合促性腺激素（FSH+ hCG或FSH+ LH）存在或不存在的情况下培养。卵母细胞进行了分析，使用传统的，数字化的，共聚焦荧光显微镜，以监测染色质，肌动蛋白和微管蛋白的组织在不同的培养条件下。大多数卵母细胞（83%）与FSH单独培养进展到中期II和停止在中期II和挤出第一个PB;相反，减数分裂进程和PB挤出受损（45-85%）在所有其他组。与卵母细胞相关的卵丘细胞的存在被认为是必要的进展到中期I和第一PB排放。在FSH单独存在下完成减数分裂成熟与卵母细胞中增强的皮质肌动蛋白聚合和含肌动蛋白的跨带卵丘过程的收缩相关。结果表明，促性腺激素产生特定的影响，减数分裂进程，PB排放，卵丘卵母细胞的相互作用，和卵母细胞的细胞骨架组织在体外成熟的仓鼠卵母细胞，表明激素控制减数分裂涉及细胞骨架的变化，在体细胞和生殖细胞。
Maturation of the mammalian oocyte involves hormone-induced meiotic cell cycle progression from prophase I to metaphase II and extrusion of the first polar body (PB). In this study, the effects of gonadotropins on meiotic cell cycle progression in cultured hamster oocytes were analyzed with respect to changes in cumulus cell-oocyte interactions and the oocyte cytoskeleton. Cumulus-oocyte complexes were obtained from large antral follicles (700 m in diameter) of eCG-primed animals and were cultured in the presence or absence of gonadotropins alone (FSH, hCG, LH) or in combination (FSH+ hCG or FSH+ LH). Oocytes were analyzed using conventional, digital, and confocal fluorescence microscopy to monitor chromatin, actin, and tubulin organization under different culture conditions. Most oocytes (83%) cultured with FSH alone progressed to and arrested at metaphase II and extruded the first PB; in contrast, meiotic progression and PB extrusion were impaired (45-85%) in all other groups. The presence of cumulus cells associated with the oocyte was found necessary for progression to metaphase I and for first PB emission. Completion of meiotic maturation in the presence of FSH alone was correlated with enhanced cortical actin polymerization in the oocyte and the retraction of actin-containing transzonal cumulus processes. The results demonstrate that gonadotropins exert specific effects on meiotic progression, PB emission, cumulus-oocyte interactions, and oocyte cytoskeletal organization during in vitro maturation of hamster oocytes, indicating that the hormonal control of meiosis involves cytoskeletal changes in both the somatic and germ cells.