Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria
Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria
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DOI：
10.1039/d3sd00049d
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发表时间：
2023-09-14
期刊：
影响因子：
--
通讯作者：
He，Xiao-Peng
中科院分区：
文献类型：
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作者：
Yan，Kai-Cheng;Thet，Naing;He，Xiao-Peng
荧光探针在生物和医学应用中被广泛使用，其功能不断增加。为了进一步解决与组织损伤相关的问题，有人提出使用长波长荧光团来构建传感器，以降低激活所需的能量并提高生物相容性。因此，我们重新设计了一种有效且生物学上适用的探针 TCF-Bpin，结合了硼酸激活基团和 TCF-OH 荧光团，用于监测细菌在抗生素治疗后产生的过氧亚硝酸盐 (ONOO−)。此外，外部添加的ONOO−在细胞内可视化，这不仅使得细菌中氧化应激的检测成为可能，而且增加了对其机制的理解。
Fluorescent probes are extensively used with ever-increasing functions for biological and medical applications. To further solve problems associated with tissue damage, long-wavelength fluorophores have been proposed for the construction of sensors with decreased energy requirements for activation and increased biocompatibility. As such we have repurposed an effective and biologically appliable probe TCF-Bpin combining boronate activating groups and a TCF-OH fluorophore, for monitoring the production of peroxynitrite (ONOO−) by bacteria upon treatment with antibiotics. In addition, externally added ONOO− was intracellularly visualized, which makes not only the detection of oxidative stress in bacteria possible but also increases the understanding of the mechanisms.