REGULATED EXPRESSION OF THE CALMODULIN-RELATED TCH GENES IN CULTURED ARABIDOPSIS CELLS - INDUCTION BY CALCIUM AND HEAT-SHOCK
REGULATED EXPRESSION OF THE CALMODULIN-RELATED TCH GENES IN CULTURED ARABIDOPSIS CELLS - INDUCTION BY CALCIUM AND HEAT-SHOCK
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DOI：
10.1073/pnas.89.8.3213
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发表时间：
1992-04-15
影响因子：
11.1
通讯作者：
BRAAM, J
中科院分区：
文献类型：
--
作者：
BRAAM, J
拟南芥中钙调蛋白相关的TCH基因在受到包括触摸在内的各种刺激后，在植物中的表达强烈而迅速地上调。为了研究TCH基因调控的机制(S)，我们建立了一个可操作的TCH基因表达调控细胞培养系统。当外界钙离子浓度升高或热休克时，TCH2、-3和-4mRNA水平显著升高。值得注意的是，这两个刺激已知会导致胞浆内钙的增加，因此暗示钙本身在钙调素相关基因的调节中发挥作用。此外，热休克最大限度地诱导TCH基因的表达是需要外部钙的，但不需要70 kDa的热休克蛋白；因此，可能存在至少两种不同的热休克诱导基因表达的机制。钙离子对钙结合蛋白编码基因的调控可能确保钙离子作为瞬时第二信使的有效性，并维持细胞内环境的稳定。这一可能的调控电路可能不仅与植物细胞有关，也与将细胞外刺激(如激素和电脉冲)转化为钙信号的各种动物细胞有关。
Expression of the calmodulin-related TCH genes of Arabidopsis is strongly and rapidly up-regulated in plants after a variety of stimuli, including touch. As an approach to investigating the mechanism(s) of TCH gene regulation, a manipulable cell culture system in which TCH gene expression is regulated has been developed. In response to increased external calcium or heat shock, TCH2, -3, and -4 MRNA levels significantly increased. Significantly, these two stimuli are known to result in cytoplasmic calcium increases, therefore implicating a role for calcium itself in the regulation of calmodulin-related genes. Further, external calcium is required for maximal heat-shock induction of expression of the TCH genes but not of the 70-kDa heat shock protein; therefore, there may exist at least two distinct mechanisms of heat shock induction of gene expression. Calcium ion regulation of genes encoding calcium-binding proteins may ensure the efficacy of calcium ion as a transient second messenger and the maintenance of cellular homeostasis. This possible regulatory circuit would likely be relevant not only for plant cells but also for the great variety of animal cells that transduce extracellular stimuli, such as hormones and electrical impulses, into calcium signals.