VISUAL PIGMENTS OF RODS AND CONES IN RHESUS-MONKEY, MACACA-MULATTA
VISUAL PIGMENTS OF RODS AND CONES IN RHESUS-MONKEY, MACACA-MULATTA
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DOI：
10.1113/jphysiol.1978.sp012151
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发表时间：
1978-01-01
影响因子：
5.5
通讯作者：
MOLLON, JD
中科院分区：
文献类型：
--
作者：
BOWMAKER, JK;DARTNALL, HJA;MOLLON, JD
用显微分光光度法对恒河猴视网膜的视杆细胞和视锥细胞的光色素进行了测定。测量光束横向穿过隔离的外段。杆状体的横向吸光度为0.02 ~ 0.04，锥状体的横向吸光度为0.01 ~ 0.03。棒材（n = 25）的平均吸光度光谱在502 +-处有一个峰值。2.7纳米。从同一组眼睛中提取的洋地黄苷给出了。lambda。最大吸收波长为499。1纳米。在82个锥体样本中，40个是红色的（P565 nm）， 42个是绿色的（P536 nm）。绿色视锥细胞的平均吸光度谱与达特纳尔图非常相似，而红色视锥细胞的平均吸光度谱较窄。没有可漂白的，蓝敏感的外部片段被记录下来，尽管发现结构被短波吸收，既不光敏也不二色性。如果假设恒河猴的长波和中波锥色素与人类相同，如果对人类外节的长度和前脑吸收作出额外的假设，就有可能得出与。pi非常相似的心理物理敏感性。5和。斯泰尔斯的4种机制。
New microspectrophotometric measurements were made of the photopigments of individual rods and cones from the retina of the rhesus monkey (M. mulatta). The measuring beam was passed transversely through isolated outer segments. The transverse absorbance for rods ranged from 0.02-0.04 and that for cones from 0.01-0.03. The mean absorbance spectrum for rods (n = 25) had a peak at 502 .+-. 2.7 nm. A digitonin extract from the same group of eyes gave a .lambda.max [wavelength of maximum absorption] of 499 .+-. 1 nm. Of a sample of 82 cones, 40 were red (P565 nm) and 42 were green (P536 nm). The mean absorbance spectrum for the green cones was very similar to the Dartnall nomogram, but that for the red cones was narrower. No bleachable, blue-sensitive outer segments were recorded, although structures were found that absorbed at short wave-lengths and were neither photosensitive nor dichroic. If the long wave length and middle wave length cone pigments of the rhesus monkey are assumed to be identical to those of man and if additional assumptions are made about the lengths of human outer segments and about prereceptoral absorption, it is possible to derive psychophysical sensitivities that closely resemble the .pi.5 and .pi.4 mechanisms of W.S. Stiles.