Structure of idiotopes associated with antiphenylarsonate antibodies expressing an intrastrain crossreactive idiotype.
Structure of idiotopes associated with antiphenylarsonate antibodies expressing an intrastrain crossreactive idiotype.
复制标题
与表达株内交叉反应独特型的抗苯胂酸抗体相关的独特位结构。
DOI：
10.1084/jem.170.4.1075
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Nisonoff,A
中科院分区：
文献类型：
--
作者：
Haba,S;Lascombe,MB;Poljak,RJ;Nisonoff,A
材料和方法单抗。所用单抗的性质概述如表I所示。CRIA‘mAb6-16和CB9在其V区部分核苷酸序列上与未突变的VH36-65胚系序列(21，22)没有不同；VH36-65及其体细胞突变体被认为编码所有CRIA’抗体(22)的V区。R16。与VH36-65编码的序列相比，VH36-65编码的序列在V段(1-98位)有3个氨基酸差异。R19.9的V片段和L链序列与VH36-65编码的序列相似。然而，它是CRIA-，可能是因为它的D区很大(11个氨基酸，而典型的CRIA‘序列中有8个氨基酸)。CRID的单抗具有与CRIA相关的V节段，但GRID与CRIA的D区和L链有明显不同，后者对应于另一个独特型家族CRIA(20)的L链。
Materials and Methods mAbs. A summary of properties of mAbs used is given in Table I. The CRIA'mAbs 6-16 and CB9 show no differences in their partial V region nucleotide sequences from the unmutated VH36-65 germline sequence (21, 22); VH36-65 and its somatic mutants are believed to encode the V region of all CRIA'antibodies (22). R16. 7, which is strongly CRIA', exhibits three amino acid differences in its V segment (positions 1-98), as compared with the sequence encoded by VH36-65 (Table I). R19. 9 has a V segment and L chain sequence similar to that encoded by VH36-65. It is however, CRIA-, probably because of the large size of its D region (11 amino acids as compared with eight in canonical CRIA'sequences). CRID'mAbs have V segments correspondingto that associated with CRIA; However, GRID differs markedly from CRIA in its D region (Table I) and L chain; the latter corresponds to the L chain of another idiotypic family, CRIA (20).