Cysteinyl leukotriene levels in esophageal mucosal biopsies of children with eosinophilic inflammation: Are they all the same?
Cysteinyl leukotriene levels in esophageal mucosal biopsies of children with eosinophilic inflammation: Are they all the same?
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DOI：
10.1111/j.1572-0241.2006.00557.x
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发表时间：
2006-05-01
影响因子：
9.8
通讯作者：
Lim, JR
中科院分区：
文献类型：
--
作者：
Gupta, SK;Peters-Golden, M;Lim, JR
目的：过敏性嗜酸性粒细胞性食管炎（AEE）以食管黏膜强烈的嗜酸性粒细胞性炎症为特征。半胱氨酸白三烯（CysLT）是一种嗜酸性化学引诱剂。我们研究了AEE患儿和对照组食管黏膜的CysLT水平。方法：采用酶联免疫吸附法（ELISA）测定内镜下食管粘膜活检组织的CysLT水平（μ g CysLT/ μ g蛋白）。结果：纳入12例急性脑梗死患儿（8例男孩，平均年龄6.6岁，范围1.0-14.5岁）和10例对照组（6例男孩，平均年龄9.56岁，范围1.08-15.08岁）。没有人接受抗肝移植或皮质类固醇治疗。所有对照组的食管、胃和十二指肠粘膜活检组织学正常。AEE患者有强烈的食管粘膜嗜酸性粒细胞炎症（平均39个/hpf，范围15-70个/hpf）， 24小时pH探针研究正常。两组之间的CysLT水平相似：AEE患者的平均水平为12.44（中位10.87，范围2.54-28.29），对照组的平均水平为9.52（中位9.26，范围1.71-21.64）。CysLT水平与食管嗜酸性粒细胞炎症程度无相关性。顺便说一句，除了食管炎外，还纳入了5例嗜酸性胃十二指肠炎患者；CysLT水平在统计学上高于对照组。结论：这是第一个在AEE患儿和正常患儿食管粘膜活检中检测CysLT水平的研究。AEE患者的CysLT水平与对照组相似，且与炎症的严重程度无关。虽然这将反对使用CysLT拮抗剂治疗AEE，但需要进一步研究CysLT受体本身的表达。
OBJECTIVES: Allergic eosinophilic esophagitis (AEE) is characterized by intense eosinophilic inflammation of the esophageal mucosa. Cysteinyl leukotrienes (CysLT) are eosinophil chemoattractants. We studied CysLT levels in esophageal mucosa of children with AEE and controls.METHODS: CysLT levels (mu g CysLT/mu g protein) were quantified by Enzyme-linked Immunosorbent Assay (ELISA) on endoscopically obtained esophageal mucosal biopsies.RESULTS: Twelve children with AEE (eight boys, mean age 6.6 yr, range 1.0-14.5 yr) and 10 controls (six boys, mean age 9.56 yr, range 1.08-15.08 yr) were enrolled. None were on anti-LT or corticosteroid therapy. All controls had histologically normal mucosal biopsies of the esophagus, stomach, and duodenum. Patients with AEE had intense eosinophilic inflammation of the esophageal mucosa (mean 39 eosinophils/hpf, range 15-70 eosinophils/hpf) and a normal 24-h pH probe study. CysLT levels were similar between the two groups: mean levels were 12.44 (median 10.87, range 2.54-28.29) in AEE patients and 9.52 (median 9.26, range 1.71-21.64) in controls. CysLT levels did not correlate with the degree of esophageal eosinophilic inflammation. Incidentally, five patients with eosinophilic gastroduodenitis, in addition to esophagitis, were enrolled; their CysLT levels were statistically higher than those of controls.CONCLUSIONS: This is the first study to examine CysLT levels in esophageal mucosal biopsies of children with AEE and normal children. CysLT levels in AEE patients are similar to those in controls, and independent of the severity of inflammation. While this would argue against the use of CysLT antagonists in the treatment of AEE, further studies into the expression of the CysLT receptor itself are needed.