Injectable and Magnetic Responsive Hydrogels with Bioinspired Ordered Structures
Injectable and Magnetic Responsive Hydrogels with Bioinspired Ordered Structures
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DOI：
10.1021/acsbiomaterials.8b01179
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发表时间：
2019-03-01
影响因子：
5.8
通讯作者：
Gomes, Manuela E.
中科院分区：
文献类型：
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作者：
Araujo-Custodio, Sandra;Gomez-Florit, Manuel;Gomes, Manuela E.
可注射水凝胶在微创组织工程和再生医学策略中的应用特别令人感兴趣。然而，这些生物材料典型的各向同性微观结构限制了它们再生有序组织的潜力。在目前的工作中，我们装饰棒形纤维素纳米晶体与磁性纳米粒子和涂层这些聚多巴胺和聚乙二醇聚合物刷，以获得化学和胶体稳定的纳米粒子。然后，将这些纳米颗粒（0.1-0.5重量%）掺入明胶水凝胶中，产生具有各种生物医学应用潜力的可注射和磁响应材料。在暴露于均匀的低磁场（108 mT）下实现水凝胶基质内的纳米颗粒对齐，从而产生具有定向微观结构和各向异性机械性能的生物材料。这些纳米复合水凝胶的生物性能进行了研究，使用脂肪组织来源的人干细胞。包封在纳米复合材料水凝胶中的细胞显示出高的存活率，证明纳米复合材料生物材料没有细胞毒性。值得注意的是，源自纳米颗粒排列的微观结构图案诱导了水凝胶中接种的和在较低程度上包封的细胞的定向生长，这表明这种可注射系统可能在靶向各向异性组织再生的细胞和非细胞策略中找到应用。
Injectable hydrogels are particularly interesting for applications in minimally invasive tissue engineering and regenerative medicine strategies. However, the typical isotropic microstructure of these biomaterials limits their potential for the regeneration of ordered tissues. In the present work, we decorated rod-shaped cellulose nanocrystals with magnetic nanoparticles and coated these with polydopamine and polyethylene glycol polymer brushes to obtain chemical and colloidal stable nanoparticles. Then, these nanoparticles (0.1-0.5 wt %) were incorporated within gelatin hydrogels, creating injectable and magnetically responsive materials with potential for various biomedical applications. Nanoparticle alignment within the hydrogel matrix was achieved under exposure to uniform low magnetic fields (108 mT), resulting in biomaterials with directional microstructure and anisotropic mechanical properties. The biological performance of these nanocomposite hydrogels was studied using adipose tissue derived human stem cells. Cells encapsulated in the nanocomposite hydrogels showed high rates of viability demonstrating that the nanocomposite biomaterials are not cytotoxic. Remarkably, the microstructural patterns stemming from nanoparticle alignment induced the directional growth of seeded and, to a lower extent, encapsulated cells in the hydrogels, suggesting that this injectable system might find application in both cellular and acellular strategies targeting the regeneration of anisotropic tissues.