Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster
Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster
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DOI：
10.1534/genetics.110.115121
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发表时间：
2010-07-01
期刊：
影响因子：
3.3
通讯作者：
Kennison, James A.
中科院分区：
文献类型：
--
作者：
Cooper, Monica T.;Conant, Alexander W.;Kennison, James A.
果蝇Chd3基因编码SNF2/RAD54 ATPase的CHD组成员。CHD蛋白从酵母到人类都是保守的，其中许多是染色质重塑复合体的亚单位，有助于转录。果蝇CHD3蛋白不是在蛋白质复合体中发现的，而是作为单体在体外重塑染色质。CHD3与延长的RNA聚合酶II共定位在唾液腺多线染色体上。由于Chd3在发育中的作用尚不清楚，我们分离并鉴定了包括Chd3在内的第三条染色体640kb区域(多线染色体区域76B-D)内的必需基因。我们恢复了24个基因的突变，这些基因对受精卵的生存至关重要。我们发现46%的必需基因的转座子插入突变体包括在果蝇基因破坏计划收集中。我们发现的所有必需基因都不在包括Chd3在内的200kb区域。我们通过靶向基因替换产生了Chd3的缺失。这一缺失对存活率或生育力都没有影响。
The Drosophila melanogaster Chd3 gene encodes a member of the CHD group of SNF2/RAD54 ATPases. CHD proteins are conserved from yeast to man and many are subunits of chromatin-remodeling complexes that facilitate transcription. Drosophila CHD3 proteins are not found in protein complexes, but as monomers that remodel chromatin in vitro. CHD3 colocalize with elongating RNA polymerase II on salivary gland polytene chromosomes. Since the role of Chd3 in development was unknown, we isolated and characterized the essential genes within the 640-kb region of the third chromosome (polytene chromosome region 76B-D) that includes Chd3. We recovered mutations in 24 genes that are essential for zygotic viability. We found that transposon-insertion mutants for 46% of the essential genes are included in the Drosophila Gene Disruption Project collection. None of the essential genes that we identified are in a 200-kb region that includes Chd3. We generated a deletion of Chd3 by targeted gene replacement. This deletion had no effect on either viability or fertility.