Interaction of granule, Purkinje and inferior olivary neurons in lurcher chimaeric mice. I. Qualitative studies.
Interaction of granule, Purkinje and inferior olivary neurons in lurcher chimaeric mice. I. Qualitative studies.
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lucher嵌合小鼠中颗粒、浦肯野神经元和下橄榄神经元的相互作用。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
K. Herrup
中科院分区：
文献类型：
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作者：
R. Wetts;K. Herrup
杂合子Lurcher(+/LC)突变小鼠失去了100%的浦肯野细胞(PC)、90%的颗粒细胞和75%的下橄榄核神经元。为了确定LC基因作用的主要部位，产生了平衡野生型聚集嵌合体。与+/LC和野生型小脑相比，三个嵌合体的小脑大小中等或正常。个人电脑的数量减少了。使用β-葡萄糖醛酸酶基因座(GUS)作为细胞标记，所有存在的PC都被鉴定为来自野生型胚胎。似乎所有+/LC PC都退化了。因此，LC基因直接作用于PC，导致其退化。嵌合体的下橄榄核神经元似乎比野生型少，但比+/LC多。β-葡萄糖醛酸酶组织化学显示，+/+和+/LC细胞均存在，其基因分型比例与脑其他区域相似。证据表明，潜伏者的橄榄核神经元的死亡是另一种缺陷的次要原因，可能是PC的丢失。β-葡萄糖醛酸苷酶不是颗粒细胞的准确细胞标记物，因此不能用这些嵌合体得出LC基因对颗粒细胞作用的结论。
Heterozygous lurcher (+/Lc) mutant mice lose 100% of their Purkinje cells (PCs), 90% of their granule cells, and 75% of their inferior olivary neurons. In order to determine the primary site of Lc gene action, lurcher in equilibrium wild-type aggregation chimaeras were produced. The cerebella of the three chimaeras examined were intermediate or normal in size compared to +/Lc and wild-type cerebella. The PCs were reduced in number. Using the beta-glucuronidase locus (Gus) as a cell marker, all of the PCs present were identified as having descended from the wild-type embryo. It appears that all of the +/Lc PCs degenerated. Hence, the Lc gene acts directly on PCs to cause their degeneration. The inferior olivary nuclei of the chimaeras seemed to have fewer neurons than wild-type but more than +/Lc animals. As revealed by beta-glucuronidase histochemistry, both +/+ and +/Lc cells were present, and the ratio of genotypes was similar to the ratio seen in other regions of the brain. The evidence suggests that the death of olivary neurons in lurcher is secondary to another defect, probably the loss of PCs. Beta-glucuronidase is not an accurate cell marker for granule cells, and so no conclusion concerning the action of the Lc gene on granule cells could be made with these chimaeras.
影响因子：
56.9
作者：
PURVES, D;LICHTMAN, JW
通讯作者：
LICHTMAN, JW