Ascorbic acid as an antioxidant in measurements of catecholamines in plasma.
Ascorbic acid as an antioxidant in measurements of catecholamines in plasma.
复制标题
抗坏血酸作为血浆儿茶酚胺测量中的抗氧化剂。
作者：
D. Hugh;A. Grennan;M. Abugila;C. Weinkove
焦亚硫酸钠通常用于防止从血浆中提取到碱性氧化铝上的过程中儿茶酚胺氧化，但不能防止其随后在乙酸洗脱液中降解。然而，抗坏血酸，一种有效的抗氧化剂，与儿茶酚胺一起提取到氧化铝上，并防止这种破坏。然而，抗坏血酸可能会干扰儿茶酚胺的电化学测量，除非使用连续的氧化和还原。最小化乙酸洗脱液中的儿茶酚胺氧化的其他方法包括在4 ° C下冷藏并将样品瓶加盖以排除大气氧气。
Sodium metabisulfite, commonly used to prevent the oxidation of catecholamines during extraction from plasma onto alkaline alumina, does not prevent their subsequent degradation in acetic acid eluates. However, ascorbic acid, a potent antioxidant, is extracted with the catecholamines onto the alumina and prevents such destruction. However, ascorbic acid may interfere with the electrochemical measurement of catecholamines, unless sequential oxidation and reduction are used. Other methods of minimizing catecholamine oxidation in acetic acid eluates include refrigerating at 4 degrees C and capping the sample vials to exclude atmospheric oxygen.