Chemical changes in cell envelope and poly-β-hydroxybutyrate during short term starvation of a marine bacterial isolate
Chemical changes in cell envelope and poly-β-hydroxybutyrate during short term starvation of a marine bacterial isolate
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DOI：
10.1007/bf00409882
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发表时间：
1986-05
影响因子：
2.8
通讯作者：
Karin Malmcrona-Friberg;A. Tunlid;P. Mårdén;S. Kjelleberg;G. Odham
中科院分区：
文献类型：
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作者：
Karin Malmcrona-Friberg;A. Tunlid;P. Mårdén;S. Kjelleberg;G. Odham
在无能量和营养的培养基中饥饿 24 小时期间，观察到海洋细菌分离物中脂质、聚-β-羟基丁酸酯 (PHB) 和细胞壁肽聚糖成分的定性和定量变化。虽然膜脂肪酸的数量和组成在饥饿的最初几个小时内波动，但脂肪酸的总量在饥饿期间下降。此外，单不饱和脂肪酸与饱和脂肪酸的比例下降，短链脂肪酸的比例增加。在饥饿的早期阶段，细菌含有在生长阶段积累的PHB，但3小时后就没有检测到PHB。缺磷的细胞表现出不同的模式，因为 PHB 最初积累，直到饥饿 5 小时才减少。细胞壁氨基酸丙氨酸的合成在饥饿的第一阶段开始。讨论了这些变化对膜流动性和底物吸收以及饥饿期间脂肪酸和 PHB 作为能源的使用的影响。
Qualitative and quantitative changes were observed in lipids, poly-β-hydroxybutyrate (PHB), and a cell wall peptidoglycan consitutent in a marine bacterial isolate during starvation for 24 h in an energy and nutrient-free medium. While the amount and composition of the membrane fatty acids fluctuated within the first hours of starvation, the total amount of fatty acids decreased during the starvation period. Furthermore, the ratio of monounsaturated to saturated fatty acids decreased and the proportion of short chain fatty acids increased. In the very early phase of starvation the bacteria contained PHB, which had been accumulated during the growth phase, but after 3 h no PHB was detected. Cells starved for phosphorus showed a different pattern as PHB was initially accumulated and did not decrease until 5 h of starvation. Synthesis of the cell wall amino acidd-alanine was initiated during the first phase of starvation. The effects of these changes on membrane fluidity and uptake of substrates as well as the use of fatty acids and PHB as energy resources during starvation are discussed.