Ex vivo detection and characterization of early dental caries by optical coherence tomography and Raman spectroscopy
Ex vivo detection and characterization of early dental caries by optical coherence tomography and Raman spectroscopy
复制标题
DOI：
10.1117/1.1915488
复制
发表时间：
2005-05-01
影响因子：
3.5
通讯作者：
Cleghorn, B
中科院分区：
文献类型：
--
作者：
Ko, ACT;Choo-Smith, LP;Cleghorn, B
早期发现龋齿将有助于非手术方法的实施，以阻止龋病进展和促进牙齿再矿化。我们提出了一种结合光学相干断层成像(OCT)和拉曼光谱的方法，以提供形态信息和生化特异性，以检测和表征在拔除的牙齿中发现的早期龋损。牙齿样本的OCT成像显示，与声音牙釉质相比，龋损部位的光后向散射强度增加。OCT图像上观察到的病变深度约为290米，与先前记录的早期龋齿相匹配。利用拉曼显微光谱学和基于光纤的拉曼光谱进一步表征龋病，观察到矿化牙齿组织中羟基磷灰石引起的PO43-振动的光谱变化。对PO43-v(2)、v(3)、v(4)振动与v(1)振动的不同比率的检查显示，与健全的牙釉质相比，龋损的发生率一致增加。这些变化归因于脱矿引起的釉质微晶形态和/或取向的改变。OCT成像可用于筛查龋齿部位和确定病变深度，而拉曼光谱可提供龋齿的生化确认。这种组合有可能发展成为一种新的光纤诊断工具，使牙医能够以更高的敏感度和特异度识别早期龋损。(C)2005年光学仪器工程师学会。
Early dental caries detection will facilitate implementation of nonsurgical methods for arresting caries progression and promoting tooth remineralization. We present a method that combines optical coherence tomography (OCT) and Raman spectroscopy to provide morphological information and biochemical specificity for detecting and characterizing incipient carious lesions found in extracted human teeth. OCT imaging of tooth samples demonstrated increased light backscattering intensity at sites of carious lesions as compared to the sound enamel. The observed lesion depth on an OCT image was approximately 290 m m matching those previously documented for incipient caries. Using Raman microspectroscopy and fiber-optic based Raman spectroscopy to characterize the caries further, spectral changes were observed in PO43- vibrations arising from hydroxyapatite of mineralized tooth tissue. Examination of various ratios of PO43- v(2), v(3), v(4) vibrations against the v(1) vibration showed consistent increases in carious lesions compared to sound enamel. The changes were attributed to demineralization-induced alterations of enamel crystallite morphology and/or orientation. OCT imaging is useful for screening carious sites and determining lesion depth, with Raman spectroscopy providing biochemical confirmation of caries. The combination has potential for development into a new fiber-optic diagnostic tool enabling dentists to identify early caries lesions with greater sensitivity and specificity. (c) 2005 Society of Photo-Optical Instrumentation Engineers.