Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells.
Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells.
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DOI：
10.1083/jcb.200105148
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发表时间：
2001-11-12
影响因子：
7.8
通讯作者：
Stossel, TP
中科院分区：
文献类型：
--
作者：
Flanagan, LA;Chou, J;Falet, H;Neujahr, R;Hartwig, JH;Stossel, TP
Arp 2/3复合物和细丝蛋白A（FLNa）分支肌动蛋白丝。为了确定这些肌动蛋白结合蛋白在细胞肌动蛋白结构中的作用，我们比较了FLNa缺陷型人黑素瘤（M2）细胞和表达正常FLNa浓度的这些细胞的三种稳定衍生物的形态。所有细胞系都含有相似量的Arp 2/3复合物。血清添加导致血清饥饿的M2细胞短暂地延伸扁平突起;此后，突起变成球形泡，并且细胞不爬行。M2细胞的短寿命lamellum包含长肌动蛋白丝的致密垫，与之相反，在相同处理的FLNa表达细胞的lamellum中，短肌动蛋白丝的三维正交网络能够平移运动。FLNa特异性抗体定位于这些细胞的整个主导层，位于正交交叉的肌动蛋白丝之间的连接处。Arp 2/3复合物特异性抗体扩散染色，并标记一些，但不相同，肌动蛋白丝重叠位点作为FLNa抗体。我们的结论是FLNa是必不可少的细胞，表达它稳定正交肌动蛋白网络适合运动。与一些建议相反，Arp 2/3复合物介导的肌动蛋白分支单独不足以建立正交肌动蛋白组织或维持前缘的机械稳定性。
The Arp2/3 complex and filamin A (FLNa) branch actin filaments. To define the role of these actin-binding proteins in cellular actin architecture, we compared the morphology of FLNa-deficient human melanoma (M2) cells and three stable derivatives of these cells expressing normal FLNa concentrations. All the cell lines contain similar amounts of the Arp2/3 complex. Serum addition causes serum-starved M2 cells to extend flat protrusions transiently; thereafter, the protrusions turn into spherical blebs and the cells do not crawl. The short-lived lamellae of M2 cells contain a dense mat of long actin filaments in contrast to a more three-dimensional orthogonal network of shorter actin filaments in lamellae of identically treated FLNa-expressing cells capable of translational locomotion. FLNa-specific antibodies localize throughout the leading lamellae of these cells at junctions between orthogonally intersecting actin filaments. Arp2/3 complex–specific antibodies stain diffusely and label a few, although not the same, actin filament overlap sites as FLNa antibody. We conclude that FLNa is essential in cells that express it for stabilizing orthogonal actin networks suitable for locomotion. Contrary to some proposals, Arp2/3 complex–mediated branching of actin alone is insufficient for establishing an orthogonal actin organization or maintaining mechanical stability at the leading edge.
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影响因子：
9.2
作者：
Bailly, M;Ichetovkin, I;Condeelis, J
通讯作者：
Condeelis, J
DOI：
10.1083/jcb.118.6.1421
发表时间：
1992-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hartwig JH
通讯作者：
Hartwig JH
DOI：
10.1083/jcb.146.6.1319
发表时间：
1999-09-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Egile C;Loisel TP;Laurent V;Li R;Pantaloni D;Sansonetti PJ;Carlier MF
通讯作者：
Carlier MF
影响因子：
7.8
作者：
SMALL, JV;HERZOG, M;ANDERSON, K
通讯作者：
ANDERSON, K
DOI：
10.1083/jcb.119.5.1219
发表时间：
1992-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lewis AK;Bridgman PC
通讯作者：
Bridgman PC