Detection and phylogeny of Staphylococcus aureus sequence type 398 in Taiwan
Detection and phylogeny of Staphylococcus aureus sequence type 398 in Taiwan
复制标题
DOI：
10.1186/s12929-019-0608-8
复制
发表时间：
2020-01-03
影响因子：
11
通讯作者：
Chen, Chih-Jung
中科院分区：
文献类型：
--
作者：
Huang, Yhu-Chering;Chen, Chih-Jung
耐甲氧西林金黄色葡萄球菌(MRSA)ST398是一种在欧洲最流行的家畜相关细菌。中国最近报道了人适应的MRSA ST398，但目前还没有台湾地区的数据。1995年至2017年在台湾收集的金黄色葡萄球菌分离株(5632株MRSA和781株敏感株)。结果共鉴定出16株ST398金黄色葡萄球菌，占0.28%，其中敏感株6株，耐药株12株。其中14株为定植株，3株为临床感染株，1株来自猪肉标本。所有3株感染株都是2015年从两名复发性中耳炎或鼻窦炎儿童中鉴定出的MSSA菌株。其他3株MSSA是在2015年从处理猪肉(2株)或猪肉(1株)的工人身上发现的。2012年，从两家养老院的居民中鉴定出了首批5株定植的MRSA菌株。2016年，从一家养老院的居民和外籍员工中分离出6株MRSA，2017年从一名外籍员工身上分离出1株MRSA。基因组序列的系统发育分析表明，所有12株地方ST398MRSA菌株都聚在一起，人类适应的，与2002年中国发现的一株人MRSA株有亲缘关系。两个本地MSSA分离株可能与家畜分离株连锁。结论金黄色葡萄球菌ST398株于2012年或更早在台湾出现。虽然有些菌株可能与家畜有关，但在台湾发现的大多数ST398金黄色葡萄球菌菌株，特别是MRSA，都是人类适应的菌株。台湾疗养院发生了人类适应的MRSA ST398菌株的本地传播，可能是从中国输入的。还需要进一步的监督。
BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) ST398 is a livestock associated-bacterium that is most prevalent in Europe. Human-adapted MRSA ST398 was recently reported from China, but there is no data available yet for Taiwan.MethodsTo identify S. aureus ST398 isolates, we examined 6413S. aureus isolates (5632 MRSA and 781 susceptible strains) that were collected in Taiwan between 1995 and 2017. If isolates could not be typed by pulsed-field gel electrophoresis upon Sma I digestion, we performed further characterization and complete genome sequencing.ResultsWe identified 18 ST398 S. aureus isolates from 16 subjects (0.28%), including 6 sensitive and 12 resistant strains. Of these, 14 were colonizing isolates, 3 were clinical (infecting) isolates and one isolate was from a pork specimen. All 3 infecting isolates were MSSA strains identified in 2015 from two children with recurrent otitis media or sinusitis. The other 3 MSSA isolates were identified from workers handling pork (2) or pork meat (1) in 2015. The first 5 MRSA colonizing isolates were identified from residents in two nursing homes in 2012. Six MRSA isolates were identified from residents and foreign employees at a nursing home in 2016 and one MRSA from a foreign worker in 2017. Phylogenetic analysis of genome sequences indicated that all 12 local ST398 MRSA strains cluster together, human-adapted and phylogenetically related to a human MRSA strain identified in China in 2002. Two local MSSA isolates could be linked to isolates from livestock. The toxin profiles were similar for the MRSA and MSSA isolates.ConclusionsOur results demonstrate that S. aureus ST398 was present in Taiwan in 2012 and potentially earlier. Although some isolates could be linked to livestock, most ST398 S. aureus isolates identified in Taiwan, particularly MRSA, represent human-adapted strains. Local transmission of human-adapted MRSA ST398 strains has occurred in nursing homes in Taiwan, possibly after import from China. Further surveillance is needed.