Structure and expression of c-myc and c-fos proto-oncogenes in thyroid carcinomas.
Structure and expression of c-myc and c-fos proto-oncogenes in thyroid carcinomas.
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DOI：
10.1038/bjc.1988.6
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发表时间：
1988-01
影响因子：
8.8
通讯作者：
Riou, G
中科院分区：
文献类型：
--
作者：
Terrier, P;Sheng, Z M;Schlumberger, M;Tubiana, M;Caillou, B;Travagli, J P;Fragu, P;Parmentier, C;Riou, G
应用Southern印迹杂交技术对23例甲状腺癌、22例甲状腺腺瘤、3例Graves病及8例正常甲状腺组织中c-myc和c-fos原癌基因的物理状态进行了分析。在4例患者中，分析了原发肿瘤和淋巴结转移。未检测到两种原癌基因的扩增或重排。还分析了总RNA。2.4kb c-myc RNA和2.2kb c-fos RNA水平升高分别见于13/23（57%）和14/23（61%）的癌症患者。高水平的c-myc转录本更常见于预后不良的甲状腺癌。同时c-myc和c-fos RNA水平升高，发现在8个癌症。22例甲状腺腺瘤、1例Graves病和2例正常甲状腺组织中均发现c-myc RNA高表达。22例甲状腺腺瘤组织中有20例c-fos RNA阳性，8例正常甲状腺组织中有2例c-fos RNA阳性。这些数据表明，c-myc和c-fos基因的过表达是独立的改变的位点。高水平的c-fos在腺瘤中发现可能与这些肿瘤的分化状态。
Tumour specimens from 23 patients with thyroid carcinoma, 22 patients with thyroid adenoma, 3 with Graves' disease, and tissues from 8 normal thyroid glands were analyzed by Southern blot hybridization for the physical state of c-myc and c-fos proto-oncogenes. In 4 patients, both the primary tumour and lymph node metastases were analyzed. No amplification or rearrangement of the two proto-oncogenes was detected. Total RNAs were also analyzed. Elevated levels of the 2.4 kb c-myc RNA and of the 2.2 kb c-fos RNA were found in 13/23 (57%) and 14/23 (61%) of the cancer patients, respectively. High levels of c-myc transcripts were more frequently found in thyroid carcinomas with unfavourable prognosis. Concomitant elevated levels of both c-myc and c-fos RNAs were found in 8 cancers. High levels of c-myc RNA were also found in 1 out of 22 specimens of adenoma, in 1 specimen of Graves' disease and in 2 normal thyroid glands. High levels of c-fos RNA were found in 20 of the 22 adenoma samples and in 2 out of 8 normal thyroid tissues. These data indicate that the overexpression of c-myc and c-fos genes is independent of an alteration of the loci. The high levels of c-fos found in adenoma may be associated with the differentiation state of these tumours.