Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in Saccharomyces cerevisiae
Acidic stress induces the formation of P-bodies, but not stress granules, with mild attenuation of bulk translation in Saccharomyces cerevisiae
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DOI：
10.1042/bj20120583
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发表时间：
2012-09-01
影响因子：
4.1
通讯作者：
Izawa, Shingo
中科院分区：
文献类型：
--
作者：
Iwaki, Aya;Izawa, Shingo
真核细胞的应激反应经常导致大量翻译活性的减弱和非翻译的mRNAs积聚成胞质mRNP(信使核糖核蛋白)颗粒，称为胞质P体(加工体)和SGS(应激颗粒)。我们研究了酸胁迫与其他形式的胁迫(如葡萄糖剥夺和酿酒酵母中的高钙水平)对mRNP颗粒形成的影响。乳酸处理明显引起P小体的形成，但不引起SGS的形成，也引起翻译起始的减弱，尽管程度比葡萄糖耗竭要小。盐酸和硫酸也能诱导P小体的形成。但在pH>3.0的SD(人工葡萄糖)介质中，乳酸、丙酸和醋酸均不能诱导P小体的形成。本研究结果表明，在低pH的外界条件下，酵母菌P小体的组装可以被诱导，其阈值在pH 2.5左右。酸性胁迫下P小体的形成需要P小体的一种成分Scd6(笼蛋白缺乏症抑制因子)，这表明酸性胁迫诱导的P小体具有不同于缺糖或高钙诱导的P小体的组装规则。
The stress response of eukaryotic cells often causes an attenuation of bulk translation activity and the accumulation of non-translating mRNAs into cytoplasmic mRNP (messenger ribonucleoprotein) granules termed cytoplasmic P-bodies (processing bodies) and SGs (stress granules). We examined effects of acidic stress on the formation of mRNP granules compared with other forms of stress such as glucose deprivation and a high Ca2+ level in Saccharomyces cerevisiae. Treatment with lactic acid clearly caused the formation of P-bodies, but not SGs, and also caused an attenuation of translation initiation, albeit to a lesser extent than glucose depletion. P-body formation was also induced by hydrochloric acid and sulfuric acid. However, lactic acid in SD (synthetic dextrose) medium with a pH greater than 3.0, propionic acid and acetic acid did not induce P-body formation. The results of the present study suggest that the assembly of yeast P-bodies can be induced by external conditions with a low pH and the threshold was around pH 2.5. The P-body formation upon acidic stress required Scd6 (suppressor of clathrin deficiency 6), a component of P-bodies, indicating that P-bodies induced by acidic stress have rules of assembly different from those induced by glucose deprivation or high Ca2+ levels.