Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis).
Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis).
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DOI：
10.1016/j.bbrc.2017.10.126
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发表时间：
2017-12
影响因子：
3.1
通讯作者：
Linna Wang;Ying Zhu;Wen‐teng Xu;C. Shao;Zhongdian Dong;Hailong Li;Zhong-kai Cui;L. Meng;Hua Guo;Yongsheng Tian;Songlin Chen
中科院分区：
文献类型：
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作者：
Linna Wang;Ying Zhu;Wen‐teng Xu;C. Shao;Zhongdian Dong;Hailong Li;Zhong-kai Cui;L. Meng;Hua Guo;Yongsheng Tian;Songlin Chen
Pod1 编码 II 类 bHLH 转录因子，参与性腺、脾脏、肺和心脏等多种组织的发育。然而，迄今为止，人们对其在硬骨鱼中的功能知之甚少。在本研究中，我们克隆并鉴定了半滑舌鱼Pod1基因。该基因包含3个外显子和2个内含子，全长cDNA为918个核苷酸，编码183个氨基酸的蛋白质，具有保守的bHLH结构域。实时定量PCR显示Pod1主要在C的睾丸中表达。 半滑鼠。在睾丸发育的不同阶段，Pod1表达在孵化后120天（dah）内检测不到，然后在孵化后210天和1年（yah）增加。此外，原位杂交（ISH）分析显示Pod1主要定位于睾丸生殖细胞，而在卵巢细胞中未检测到；这表明它在 C 的精子发生中可能具有功能。 半滑鼠。性腺Pod1基因组序列甲基化谱分析表明，卵巢、雄性和假雄性睾丸中Pod1的推定启动子区域差异不明显。因此，可能需要进一步的研究来评估Pod1表达是否受到表观遗传水平的调节。
Pod1encodes a Class II bHLH transcription factor involved in the development of a number of tissues such as gonad, spleen, lungs and heart. However, to date, little is known about its function in teleosts. In this study, we cloned and characterizedPod1gene fromCynoglossus semilaevis. This gene contains three exons and two introns, with the full-length cDNA of 918 nucleotides that encodes a 183 amino acid protein with a conserved bHLH domain. Realtime quantitative PCR revealed thatPod1was predominantly expressed in the testes ofC. semilaevis. In different stages of testes development,Pod1expression was undetectable up to 120 days after hatching (dah), and then increased at 210 dah and 1 year after hatching (yah). Furthermore,in situhybridization (ISH) analysis revealed thatPod1was mainly localized in the germ cells of testes, but was not detected in ovarian cells; which suggested its possible functions in spermatogenesis ofC. semilaevis. The methylation profile analysis ofPod1genomic sequence in the gonads showed that the differences in their putative promoter regions ofPod1among ovary, male and pseudo-male testes were not obvious. Thus, further research might be needed to evaluate whetherPod1expression is regulated by epigenetic level.