Slowing DNA Translocation through Nanopores Using a Solution Containing Organic Salts
Slowing DNA Translocation through Nanopores Using a Solution Containing Organic Salts
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DOI：
10.1021/jp9040293
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发表时间：
2009-10-08
影响因子：
3.3
通讯作者：
Guan, Xiyun
中科院分区：
文献类型：
--
作者：
de Zoysa, Ranulu Samanthi S.;Jayawardhana, Dilani A.;Guan, Xiyun
纳米孔 DNA 测序的关键挑战之一是减慢 DNA 易位。在这里，我们报告说，通过使用含有有机盐的电解质溶液，可以显着降低各种 DNA 均聚物和共聚物通过蛋白质孔的易位速度。使用氯化丁基甲基咪唑鎓 (BMIM-Cl) 溶液代替常用的 KCl 溶液，实现了每个核苷酸碱基数百微秒数量级的 DNA 易位速率。纳米孔分辨率大大提高，加上不同核苷酸产生的不同事件阻断幅度，可以方便地区分各种 DNA 分子。
One of the key challenges to nanopore DNA sequencing is to slow down DNA translocation. Here, we report that the translocation velocities of various DNA homo- and copolymers through protein pores could be significantly decreased by using electrolyte Solutions containing organic salts. Using a butyl-methylimidazolium chloride (BMIM-Cl) Solution instead of the commonly used KCl solution, DNA translocation rates on the order of hundreds of microseconds per nucleotide base were achieved. The much enhanced resolution of the nanopore coupled with different event blockage amplitudes produced by different nucleotides permits the convenient differentiation between various DNA molecules.