Super-SILAC mix for quantitative proteomics of human tumor tissue
Super-SILAC mix for quantitative proteomics of human tumor tissue
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DOI：
10.1038/nmeth.1446
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发表时间：
2010-05-01
期刊：
影响因子：
48
通讯作者：
Mann, Matthias
中科院分区：
文献类型：
--
作者：
Geiger, Tamar;Cox, Juergen;Mann, Matthias
我们描述了一种通过将五种稳定同位素标记的氨基酸标记细胞培养(SILAC)标记的细胞系与人类肿瘤组织相结合来准确定量人类肿瘤蛋白质组的方法。这产生了数十万个适量的同位素标记的多肽，作为基于质谱学分析的内部标准。通过将标记与测量分离，这种超SILAC方法扩大了基于SILAC的蛋白质组学的范围。
We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.