Molecular cloning and sequencing of cytochrome c' from the phototrophic purple sulfur bacterium Chromatium vinosum.
Molecular cloning and sequencing of cytochrome c' from the phototrophic purple sulfur bacterium Chromatium vinosum.
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光养紫色硫细菌 Chromatium vinosum 细胞色素 c 的分子克隆和测序。
DOI：
10.1016/0005-2728(95)00101-n
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Cusanovich,MA
中科院分区：
文献类型：
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作者：
Even,MT;Kassner,RJ;Dolata,M;Meyer,TE;Cusanovich,MA
该基因是从HindIII-Sali基因组DNA中克隆得到的。用Sanger双脱氧法对含有该基因的1.4kbP片段进行双向测序。细胞色素c‘基因编码154个残基的多肽，其中最后131个氨基酸与先前确定的蛋白质序列相匹配。剩下的23个残基代表一个信号序列，该序列在转位到周质间隙时从多肽中切割出来。在片段的另一条链上的另一个开放阅读框编码包含与几个镍铁氢酶的细胞色素b型亚单位的相应区域同源的四个区域的肽。
The gene for cytochrome c′ from Chromatium vinosum was cloned from a HindIII-SalI digest of genomic DNA. A 1.4 kbp fragment containing the gene was sequenced in both directions using the Sanger dideoxy method. The cytochrome c′ gene codes for a 154-residue peptide, of which the last 131 amino acids match the previously determined sequence of the protein. The remaining 23 residues represent a signal sequence that is cleaved from the polypeptide upon translocation to the periplasmic space. An additional open reading frame on the other strand of the fragment codes for a peptide that contains four regions that are homologous to corresponding regions of the cytochrome b-type subunit of several Ni-Fe hydrogenases.