CHOLINERGIC AMACRINE CELLS OF THE RABBIT RETINA CONTAIN GLUTAMATE-DECARBOXYLASE AND GAMMA-AMINOBUTYRATE IMMUNOREACTIVITY
CHOLINERGIC AMACRINE CELLS OF THE RABBIT RETINA CONTAIN GLUTAMATE-DECARBOXYLASE AND GAMMA-AMINOBUTYRATE IMMUNOREACTIVITY
复制标题
DOI：
10.1073/pnas.85.16.6187
复制
发表时间：
1988-08-01
影响因子：
11.1
通讯作者：
WASSLE, H
中科院分区：
文献类型：
--
作者：
BRECHA, N;JOHNSON, D;WASSLE, H
乙酰胆碱和γ-氨基丁酸(GABA)递质在视网膜神经节细胞感受野特性的形成中起着关键作用。在兔视网膜，胆碱能无长突细胞和移位的无长突细胞通过胆碱乙酰转移酶的免疫组织化学染色和荧光染料4‘’，6-二氨基-2-苯吲哚的大量积聚来识别。还鉴定了几种GABA免疫反应阳性和谷氨酸脱羧酶免疫反应阳性的细胞类型，包括大量小的圆形无长突细胞和移位的无长突细胞。双标记实验表明，所有显著积聚4‘’，6-二氨基-2-苯基吲哚的无长突细胞和移位的无长突细胞均含有GABA和谷氨酸脱羧酶免疫反应。然而，并不是所有的GABA免疫反应细胞都积累了这种染料。对视网膜整体贴片的神经节细胞层的定量分析表明，胆碱乙酰转移酶免疫反应细胞和大多数GABA免疫反应细胞具有小而圆的形状和与移位的无长突细胞相似的细胞密度分布。这些研究证实，胆碱能细胞是GABA免疫反应的无长突细胞和移位的无长突细胞的主要亚群。这些细胞在神经节细胞感受野特性的形成中所起的作用可以简单地解释为乙酰胆碱介导的兴奋性突触后作用和GABA介导的抑制性突触前作用。
The transmitters acetylcholine and .gamma.-aminobutyrate (GABA) play critical roles in the formation of receptive-field properties of retinal ganglion cells. In rabbit retina, cholinergic amacrine and displaced amacrine cells were identified by immunohistochemical staining for the enzyme choline acetyltransferase and by their avid accumulation of the fluorescent dye 4'', 6-diamidino-2-phenylindole. Several GABA-immunoreactive and glutamate decarboxylase-immunoreactive cell types, including a prominent population of small, round amacrine and displaced amacrine cells, were also identified. Double-label experiments demonstrated that all amacrine and displaced amacrine cells that prominently accumulate 4'',6-diamidino-2-phenylindole contain GABA and glutamate decarboxylase immunoreactivity. However, not all GABA-immunoreactive cells accumulate this dye. Quantitative analysis of the ganglion cell layer of whole mount preparations of the retina showed that choline acetyltransferase-immunoreactive cells and the majority of GABA-immunoreactive cells have a small, round shape and similar cell density profiles that parallel that of displaced amacrine cells. These studies establish that cholinergic cells are a major subpopulation of GABA-immunoreactive amacrine and displaced amacrine cells. The role these cells have in the formation of ganglion cell receptive-field properties may be parsimoniously explained by an excitatory postsynaptic action mediated by acetylcholine and an inhibitory presynaptic action mediated by GABA.