Tissue engineering strategy using mesenchymal stem cell-based chitosan scafolds in growth plate surgery: A preliminary study in rabbits
Tissue engineering strategy using mesenchymal stem cell-based chitosan scafolds in growth plate surgery: A preliminary study in rabbits
复制标题
DOI：
10.1016/j.otsr.2015.04.010
复制
发表时间：
2015-09-01
影响因子：
2.3
通讯作者：
Geramizadeh, B.
中科院分区：
文献类型：
--
作者：
Azarpira, M. R.;Shahcheraghi, G. H.;Geramizadeh, B.
背景：儿童生长板损伤可能导致肢体长度差异和成角畸形。去除受损的骺板或骨棒并插入垫片会产生不同的结果，对于幼儿的大缺陷，治疗具有挑战性。在这项研究中，我们使用组织工程间充质干细胞（基于 MSC 的壳聚糖支架）来修复受损的骺板。还研究了壳聚糖作为间隔物的使用。材料和方法：通过切除14只4周龄白化兔外侧50%的股骨远端骨骺来建立生长停滞的实验模型。 10只兔子的左侧生长板缺损用基于MSC的壳聚糖支架填充，4只兔子的左侧生长板缺陷仅填充支架。对于所有兔子，右侧缺陷被单独保留作为对照肢体。 3个月后，取出股骨并进行肉眼检查和放射学测量角度；还进行了评估骺再生的组织学研究。结果：半骺切除手术成功，所有手术肢体均出现成角畸形。在使用更多浓度的带有壳聚糖支架的 MSC 的情况下，有减少角度畸形的趋势。在转移浓度低于150万个MSC的情况下，观察到混合结果并且角畸形没有减少。单独移植壳聚糖效果不佳。结论：在本研究中，我们使用天然壳聚糖支架和 MSC 开发了一种可移植组织工程盘的体外构建方法。我们研究了这些椎间盘修复股骨远端生长板软骨缺陷的功效。我们的结果表明，这些细胞对支架的有益作用出现在更多浓度的细胞中。证据级别：III级。低功耗对比研究。 (C) 2015 年由 Elsevier Masson SAS 出版。
Background: Growth plate injury in children could produce limb length discrepancy and angular deformity. Removal of damaged physis or bony bar and insertion of spacers produced variable results and for large defects in young children, the treatment is challenging. In this study, we used tissue-engineered mesenchymal stem cells (MSC-based chitosan scaffold) for restoration of the damaged physis. The usage of chitosan as a spacer was also investigated.Materials and methods: An experimental model of growth arrest was created by removing lateral 50% of distal femoral physis of fourteen 4-week-olds albino rabbits. The left side growth plate defects were filled with MSC-based chitosan scaffold in 10 and scaffold alone in 4 rabbits. For all the rabbits, right-side defects were left alone as the control limb. After 3 months, femoral bones were harvested and gross inspection and radiology for measurement of angulations were done; histological study for evaluation of regeneration of physis was also done.Results: The hemiphyseal resection procedures were successful and all of the operated limbs showed angular deformities. There was a trend toward less angular deformity in cases in which more concentration of MSCs with chitosan scaffold was used. In cases of transfer of MSCs with concentration of less than 1.5 millions, mixed results were observed and angular deformities were not reduced. Transfer of chitosan alone yielded poor results.Conclusion: In this study, we have developed an in vitro construction of a transplantable tissue-engineered disk, using natural chitosan scaffold and MSCs. We investigated the efficacy of these disks for repairing the defect of growth plate cartilage at distal femoral physis. Our results showed that the beneficial effect of these cells on scaffold appeared in more concentration of cells. Level of evidence: Level III. Low power comparative study. (C) 2015 Published by Elsevier Masson SAS.