COMPARATIVE USE OF FRUCTOSE AND GLUCOSE IN HUMAN-LIVER AND FIBROBLASTIC CELL-CULTURES
COMPARATIVE USE OF FRUCTOSE AND GLUCOSE IN HUMAN-LIVER AND FIBROBLASTIC CELL-CULTURES
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DOI：
10.1007/bf02618875
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
LEMONNIER, A
中科院分区：
文献类型：
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作者：
DELHOTAL, B;LEMONNIER, F;LEMONNIER, A
以人皮肤成纤维细胞和肝细胞为实验材料，研究了果糖在细胞培养液中替代葡萄糖的作用。细胞在4种不同的培养基中培养2~10d，分别含有5.5mmol.1mmol.1−1和27.5mmol.i−1葡萄糖和果糖。在果糖存在的情况下，细胞生长受到刺激，但在肝细胞中的作用低于成纤维细胞。在第6天，观察到[~3H]胸腺嘧啶核苷掺入、蛋白质水平和氨基酸消耗增加，而ATP水平下降。在含有5.5mmol.1−1葡萄糖或果糖的培养基中，果糖的消耗量在第3天比葡萄糖低4倍，直到第6天才开始上升。在果糖中，乳酸的产量很低(比葡萄糖低4~5倍)，pH值始终较高。对于成纤维细胞和肝细胞，由于这两种细胞在培养中的特殊性，有些结果是不同的，尤其是在葡萄糖和果糖浓度为27.5mmol.1−1的情况下，这一点尤其明显。
The effect of fructose as a substitute for glucose in cell culture media was investigated in human skin fibroblast and liver cell cultures. Cells were grown for between 2 and 10 days in identical flasks in four different media, containing 5.5, mmol·1−1and 27.5 mmol·I−1glucose and fructose, respectively. In the presence of fructose, cell growth was stimulated, but less in liver cells than fibroblasts. At Day 6, increases were observed in [3H]thymidine incorporation, protein levels, and amino acid consumption, and a reduction was noted in ATP levels. In media containing 5.5, mmol·1−1glucose or fructose, consumption of fructose was four times lower than that of glucose at Day 3 and did not rise until Day 6. In fructose media, the lactate production was very low (four to five times less than that of glucose) and the pH values were always higher. Some findings were different for the fibroblasts and liver cells, owing to the specific characteristics of these two cell types in culture; this applied especially to the effects of glucose and fructose concentrations of 27.5 mmol·1−1. Several possible explanation for the stimulation of cell growth in fructose medium were discussed.