Headset bio-sniffer with wireless CMOS camera for percutaneous ethanol vapor from the ear canal
Headset bio-sniffer with wireless CMOS camera for percutaneous ethanol vapor from the ear canal
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带有无线 CMOS 摄像头的耳机生物嗅探器，用于从耳道采集经皮乙醇蒸气
DOI：
10.1016/j.biosx.2022.100169
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发表时间：
2022
影响因子：
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通讯作者：
Mitsubayashi Kohji
中科院分区：
文献类型：
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作者：
Arakawa Takahiro;Ishikawa Riki;Iitani Kenta;Toma Koji;Iwasaki Yasuhiko;Mitsubayashi Kohji
人体挥发物中含有的挥发性有机化合物与新陈代谢和疾病有关。这些成分的测量有望实现非侵入性和简单的代谢评估和疾病诊断。特别是，可以不受限制地连续收集皮肤气体，这是下一代可穿戴测量设备的有用特性。在这项研究中，我们通过构建与互补金属氧化物半导体（CMOS）相机集成的耳机式无限制耳道气体测量系统，开发了一种可穿戴的乙醇生化气体传感器（biosniffer）。该系统通过检测乙醇脱氢酶（ADH）氧化乙醇过程中产生的NADH荧光的增加来测量皮肤释放的乙醇气体。在该系统中，来自UV-LED的激发光通过带通滤波器照射ADH酶固定化膜，并且NADH的荧光由具有Wi-Fi功能的小型CMOS相机检测。该系统集成到耳罩中，形成可穿戴耳机。荧光图像显示与乙醇气体浓度相关的增加的输出。当使用积分分析来评估荧光的增加时，可以测量11 ppb-444 ppm的乙醇气体，该范围包括施用酒精后皮肤中的乙醇浓度。此外，当将该装置附接到正在饮酒的人类参与者时，确认了基于饮酒和代谢的输出的增加和减少，这表明可以在汗液影响最小的情况下直接测量来自耳道的乙醇气体。
Volatile organic compounds contained in human volatiles are related to metabolism and disease. Measurement of these components is expected to enable non-invasive and simple metabolic evaluations and disease diagnoses. In particular, skin gas can be collected continuously without constraints, a useful property for the next generation of wearable measurement devices. In this study, we develop a wearable biochemical gas sensor (biosniffer) for ethanol by constructing a headset-type unrestrained ear canal gas measurement system integrated with a complementary metal-oxide semiconductor (CMOS) camera. This system measures ethanol gas released by the skin by detecting the increase in fluorescence of NADH, which is produced during the oxidation of ethanol by alcohol dehydrogenase (ADH). In this system, excitation light from a UV-LED irradiates an ADH enzyme-immobilized membrane through a band-pass filter, and the fluorescence of NADH is detected by a small CMOS camera with Wi-Fi functionality. This system is integrated into an earmuff, forming a wearable headset. Fluorescence images shows an increased output correlated with the ethanol gas concentration. When integral analysis is used to assess the increase in fluorescence, it is possible to measure ethanol gas at 11 ppb–444 ppm, a range that includes the ethanol concentration in the skin after alcohol administration. Furthermore, when this device is attached to a human participant who is consuming alcohol, the increase and decrease output based on drinking and metabolism is confirmed, indicating the possibility of directly measuring ethanol gas from the ear canal with minimal effect of perspiration.