Regional expression of the transcript encoding sterol carrier protein x-related thiolase and its regulation by homeotic genes in the midgut of Drosophila embryos
Regional expression of the transcript encoding sterol carrier protein x-related thiolase and its regulation by homeotic genes in the midgut of Drosophila embryos
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DOI：
10.1046/j.1440-169x.1996.t01-3-00005.x
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发表时间：
1996-08-01
影响因子：
2.5
通讯作者：
Okada, M
中科院分区：
文献类型：
--
作者：
Kitamura, T;Kobayashi, S;Okada, M
我们已经分离出一个基因指定的终端环中肠（修剪），它编码的转录本中表达的胚胎中肠在一个区域特异性的方式。1.4 kb的修剪转录表达在两个离散区域的中肠上皮细胞在胚胎发育后期。TRIM表达的前域位于前胃和第一中肠缩窄部之间。后区位于第三中肠缩窄部的后方。此外，我们表明，正常功能的两个同源异型基因，Antp和abd-A，这在内脏中胚层覆盖中肠上皮细胞的表达，是必要的区域表达修剪。因此，trim基因被认为是通过两个中肠层之间的诱导相互作用而被激活的。1.4kb的mRNA编码固醇载体蛋白X相关的硫解酶。由于硫解酶活性是脂质代谢所必需的，因此1.4kb修剪转录物的表达可以代表分化状态，并且可以用作分析中肠在生理学上不同的结构域方面的区域分化的适当标记。
We have isolated a gene designated terminal rings in the midgut (trim), which encodes a transcript expressed in the embryonic midgut in a region-specific manner. The 1.4 kb trim transcript is expressed in two discrete regions of the midgut epithelium during late embryogenesis. The anterior domain of trim expression is found between the proventriculus and the first midgut constriction. The posterior domain locates in the region posterior to the third midgut constriction. Furthermore, we show that normal function of two homeotic genes, Antp and abd-A, which express in the visceral mesoderm covering the midgut epithelium, is required for the regional expression of trim. The trim gene is thus reasoned to be activated through inductive interaction between two midgut layers. The 1.4 kb mRNA encodes a sterol carrier protein x-related thiolase. As thiolase activity is required for lipid metabolism, the expression of the 1.4 kb trim transcript can represent a state of differentiation and can be used as an appropriate marker for analyzing regional differentiation of the midgut in terms of physiologically distinct domains.