Analysis of an expression profile of genes in the human adipose tissue
Analysis of an expression profile of genes in the human adipose tissue
复制标题
DOI：
10.1016/s0378-1119(96)00730-5
复制
发表时间：
1997-05-06
期刊：
影响因子：
3.5
通讯作者：
Matsubara, K
中科院分区：
文献类型：
--
作者：
Maeda, K;Okubo, K;Matsubara, K
越来越多的证据表明，除了将多余的能量储存为脂肪外，脂肪组织还充当内分泌器官，将各种因子分泌到血流中。然而，每当在脂肪组织中发现一个新的因子时，其含义都是独立讨论的，并且还没有进行基于该组织基因表达的全局观点的系统分析。为了描述该组织在基因表达方面的功能，并寻找新的因子，我们使用忠实地代表信使RNA（mRNA）组成的3 '定向cDNA文库进行随机互补DNA（cDNA）测序。各种众所周知但意想不到的基因，包括凝溶胶蛋白，血浆谷胱甘肽过氧化物酶（GPX-3）和羧肽酶E（CPE）的基因被证明非常活跃。通过比较脂肪中活性基因的表达谱与其他组织中的活性基因的表达谱以及dbEST中的数据，我们确定了7个在脂肪组织中特异性表达的新基因。其中，一个编码的蛋白质与胶原样重复和一个假定的分泌信号。这些数据可用作分析该组织的生理学以及肥胖的病因学和并发症的新工具。
Increasing evidence suggests that in addition to storing excess energy as fat, adipose tissue acts as an endocrine organ secreting various factors into the blood stream. Every time a new factor is found in adipose tissue, however, its implication is discussed independently, and a systematic analyses based upon a global view of gene expression of this tissue has not been performed. To describe the function of this tissue in terms of gene expression, and to find new factors, we performed random complementary DNA (cDNA) sequencing using a 3'-directed cDNA library that faithfully represents the composition of the messenger RNA (mRNA). Various well-known but unexpected genes, including those for gelsolin, plasma glutathione peroxidase (GPX-3) and carboxypeptidase E (CPE) were shown to be very active. By comparing the expression profile of active genes in the adipose with those of other tissues and with data in dbEST, we identified seven new genes that are specifically expressed in adipose tissue. Among these, one encoded a protein with collagen-like repeats and a putative secretion signal. These data can be used as new tools for analyses of the physiology of this tissue, as well as the etiology and complications of obesity.