Measurement of virulence in Zymoseptoria tritici through low inoculum-density assays.
Measurement of virulence in Zymoseptoria tritici through low inoculum-density assays.
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DOI：
10.1016/j.fgb.2015.03.020
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Gurr SJ
中科院分区：
文献类型：
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作者：
Fones HN;Steinberg G;Gurr SJ
迄今为止，用高密度接种物接种宿主叶子后，对小麦发酵斑孢菌突变体或野生型变体的致病性测定一直基于分生孢子器计数。在这里，我们提供的数据表明，高接种密度可能掩盖由于症状饱和而导致的毒力缺陷。我们描述了一种低接种密度方法，可以避免这个问题。该方法还可用于（i）询问小麦病灶形成的过程（ii）确定相同或不同基因型的个体在质外体感染的建立过程中是否合作或竞争（iii）通过评估给定菌株的气孔渗透效率（SPE）、其在质外体中传播的能力及其分生效率来剖析毒力的决定因素。因此，这种方法可用于研究突变型或无毒力野生型菌株毒力减弱的原因。
Hitherto, pathogenicity assays with mutants or wildtype variants of Zymoseptoria tritici have been based on pycnidial counts, following inoculation of host leaves with high density inoculum. Here, we present data which suggest that high inoculum densities may mask deficiencies in virulence due to symptom saturation. We describe a low inoculum-density method which obviates this problem. This method can also be used to (i) interrogate the process of lesion formation in Z. tritici (ii) determine whether individuals of the same or different genotypes co-operate or compete during the establishment of apoplastic infections (iii) dissect the determinants of virulence, by assessing a given strain’s stomatal penetration efficiency (SPE), its ability to spread within the apoplast and its pycnidiation efficiency. Such methodology can thus be used to investigate the reasons underpinning attenuated virulence in mutant or avirulent wildtype strains.